The analysis of molecular mechanism of bone destruction using invitro cocultivation assay and application to a risk management in ameloblastoma

2013 Fiscal Year Final Research Report

• Project/Area Number: 24659900
• Research Category: Grant-in-Aid for Challenging Exploratory Research
• Allocation Type: Single-year Grants
• Research Field: Surgical dentistry
• Research Institution: Kagoshima University
• Principal Investigator: FUJII Tomomi 鹿児島大学, 医歯(薬)学総合研究科, 教務職員 (90305152)
• Co-Investigator(Kenkyū-buntansha): KISHIDA Shosei 鹿児島大学, 医歯学総合研究科, 教授 (50274064) ; SENBA Ichiro 鹿児島大学, 医歯学総合研究科, 教授 (60145505) ; NAKAMURA Norifumi 鹿児島大学, 医歯学総合研究科, 教授 (60217875) ; KOMATSUZAWA Hitoshi 鹿児島大学, 医歯学総合研究科, 教授 (90253088) ; KIBE Toshiro 鹿児島大学, 医学部歯学部附属病院, 医員 (50635480)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 国際情報交換 / インドネシア / エナメル上皮腫

Research Abstract

In this study, we immortalized cells which we obtained from the lesion of ameloblastoma and the oral mucosa of the healthy subject and established new ameloblastoma cell line (AM-3) and normal oral mucosa epithelium cell line (MOE-1). The expression of Wnt5a, Fz-2, MMP-2, -9 was higher remarkably in ameloblastoma cell line than normal oral mucosa epithelium cell line. Also, Wnt-3a treatment activated expression of MMP-9 of AM-3. Furthermore, osteoclasts differentiation was induced when we cocultivated with AM-3cells and RAW 264.7 cells which were osteoclastic precursors.

Research Products

• [Journal Article] Immunoreactivity of Wnt5a, Fzd2, Fzd6, and Ryk in glioblastoma : evaluative methodology for DAB chromogenic immunostaining (2014)
  • Author(s): Hirano H, Yonezawa H, Yunoue S, Habu M, Uchida H, Yoshioka T, Kishida S, Kishida M, Oyoshi T, Fujio S, Sugata S, Yamahata H, Hanaya R, Arita K
  • Journal Title: Brain Tumor Pathol Volume: 31(2) Pages: 85-93
  • DOI: 10.1007/s10014-013-0153-1
  • Peer Reviewed
• [Journal Article] Ryk is essential for Wnt-5a-dependent invasiveness in human glioma (2014)
  • Author(s): Habu M, Koyama H, Kishida M, Kamino M, Iijima M, Fuchigami T, Tokimura H, Ueda M, Tokudome M, Koriyama C, Hirano H, Arita K, Kishida S
  • Journal Title: J Biochem Volume: (in press)
  • Peer Reviewed
• [Journal Article] A novel ameloblastoma cell line (AM3) secretes MMP-9 in response to Wnt-3a and induces osteoclastgenesis (2013)
  • Author(s): Toshiro Kibe, Takao Fuchigami, Michiko Kishida, Mikio Iijima, Hiroshi Hijioka, Akihiko Miyawaki, Ichiro Senba, Tooru Kiyono, Norifumi Nakamura, Shosei Kishida
  • Journal Title: Oral Surg Oral Med Oral Pathol Oral Radiol Endod Volume: 115(6) Pages: 780-788
  • DOI: 10.1016/j.oooo.2013.03.005.
  • Peer Reviewed
• [Presentation] エナメル上皮腫由来細胞株の浸潤能におけるWntシグナルの意義 (2012)
  • Author(s): 岐部俊郎、岸田昭世、比地岡浩志、宮脇昭彦、中村典史
  • Organizer: 第66回NPO法人日本口腔科学会学術集会
  • Place of Presentation: 広島市
  • Year and Date: 20120517-18