DEVELOPMENT OF EFFECTIVE PURIFICATION METHOD FOR WATER ENVIRONMENT POLLUTED BY CHEMICAL SUBSTRATES HIGHLY APPLYING RHIZOSPHERE EFFECT OF AQUATIC PLANTS

2014 Fiscal Year Final Research Report

• Project/Area Number: 24681011
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Environmental technology/Environmental materials
• Research Institution: Kitasato University
• Principal Investigator: SEI KAZUNARI 北里大学, 医療衛生学部, 教授 (80324177)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 環境保全技術 / 根圏浄化法 / 水生植物 / 根圏微生物 / 発現解析 / 微量汚染化学物質

Outline of Final Research Achievements

Gene expression analyses of Sphingobium fuliginis OMI, isolated from the rhizosphere of Spirodela polyrrhiza (giant duckweed), were performed using DNA microarray to clarify the mechanism of specific enrichment of this microbe in the rhizosphere of duckweeds.
Although most of the strongly expressed genes of strain OMI were related to metabolisms of substrates and transcriptional regulators, the genes related to synthesize chemotaxis protein and type IV pilus were unique during the symbiotic cultivation with duckweeds. The type IV pilus is reported to act as an anchor to attach to a surface of a certain host. Thus, one of the possible mechanisms for the selective enrichment of strain OMI by duckweeds is the induction of genes to synthesize chemotaxis protein, which allows strain OMI to move to the rhizosphere of duckweeds, and the induction of genes to synthesize type IV pilus, which allows strain OMI to attach onto the root surface of the duckweeds.

Free Research Field

生物環境工学