Highly efficient cell-free membrane protein expression in organic-inorganic hybrid vesicles

2015 Fiscal Year Final Research Report

• Project/Area Number: 24681028
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Microdevices/Nanodevices
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: Yasuhara Kazuma 奈良先端科学技術大学院大学, 物質創成科学研究科, 助教 (90545716)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: バイオリアクター / マイクロ・ナノデバイス / 有機－無機ハイブリッド / ベシクル / 遺伝子発現

Outline of Final Research Achievements

This study focused on the development of a highly efficient cell-free membrane protein expression system using organic-inorganic hybrid vesicles. The organic-inorganic hybrid vesicles with a crosslinked ceramic-like surface have a semi-permeable feature in which small molecules can permeate across the membrane whereas large molecules cannot. The structure and semi-permeable feature of the organic-inorganic hybrid vesicles can be tuned by the incorporation of various amphiphiles. It was suggested that the encapsulation of enzymatic reaction system enables the development of a membrane protein expression system, which utilizes the semi-permeable feature of the organic-inorganic hybrid vesicles.

Free Research Field

界面物理化学、高分子化学