Search for peptide-biosynthetic genes using hydroxy amino acid tracers

2014 Fiscal Year Final Research Report

• Project/Area Number: 24688010
• Research Category: Grant-in-Aid for Young Scientists (A)
• Allocation Type: Partial Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Kyoto University
• Principal Investigator: HIBI Makoto 京都大学, (連合)農学研究科(研究院), 助教 (30432347)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 水酸化アミノ酸 / ペプチド生合成 / アミノ酸変換酵素

Outline of Final Research Achievements

By the gene cluster analysis method based on amino acid hydroxylase (DO), biosynthetic pathway elucidation of novel peptide molecular species was carried out. A partial peptide biosynthetic pathway was revealed by functional analysis of each constitutive enzyme in non-ribosomal peptide synthesis (NRPS) cluster including a DO gene found in the genome of Burkholderia ambifaria AMMD. Also, novel DOs were found from filamentous fungi to generate hydroxy cyclic amino acids, which often constitute peptides, and were applied to detailed enzymatic characterization analysis. Taking advantage of these results, isotopically labeled hydroxy amino acids were successfully produced by enzyme catalytic processes, and were it possible to trace microbial metabolic intermediates in peptide biosynthesis.

Free Research Field

応用微生物学