2014 Fiscal Year Final Research Report
Biological functions of ultradian oscillation revealed by bioluminescence and fluorescence imaging
| Project/Area Number |
24700293
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bioinformatics/Life informatics
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | システム生物学 |
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| Outline of Final Research Achievements |
We tought to visualize genetic oscillation and myogenic differentiation process simultaneously in myoblast cells by combining fluorescence and bioluminescence imaging tequniques. We succeeded in the visualization of Hes1 oscillatory dynamics in myogenic differentiation, but we did not find correlation between ultradian dynamics and differentiation states. On the other hand, we also developed infrared fluorecent reporter of cell cycle progression and optogenetic tools to control gene expression dynamics in a precise temporal manner.
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| Free Research Field |
生物物理学
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