2013 Fiscal Year Final Research Report

Functional analysis of the role of hippocampal CA2 region in memory by conditional targeting

Research Project

Project/Area Number	24700321
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Neuroscience in general
Research Institution	Niigata University
Principal Investigator	ABE Manabu 新潟大学, 脳研究所, 准教授 (10334674)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	海馬 / コンディショナルジーンターゲティング法 / 記憶
Research Abstract	The function of the hippocampal CA2 region remains largely unknown. The aim of this study was to examine the role of CA2 neurons in memory by conditional targeting. To develop a CA2 neuron-selective gene targeting system, we made several knock-in lines, in which Cre recombinase or tetracycline transactivator was inserted into the translational initiation site of the target genes (Cacng5, Rgs14, Abat and so on) in frame. The genes seem to be strongly expressed in the CA2 region. Additionally, we made a new line expressing diphteria toxin receptor under control of the activity of Cre recombinase or the induction of tetracycline transactivator. The mutant lines developed in this study could be useful tools for functional analysis of hippocampus-dependent memory.

(5 results)