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2013 Fiscal Year Final Research Report

The regulatory mechanisms and the physiological significance of SNAP-25 phosphorylation in mouse brain.

Research Project

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Project/Area Number 24700385
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Neurochemistry/Neuropharmacology
Research InstitutionKitasato University

Principal Investigator

YAMAMORI Saori  北里大学, 医学部, 講師 (30464803)

Project Period (FY) 2012-04-01 – 2014-03-31
Keywordsリン酸化 / 神経伝達物質放出 / ストレス / SNAREタンパク質 / プレシナプス / ドーパミン / SNAP-25 / PKC
Research Abstract

SNAP-25 plays a crucial role in neurotransmitter release by exocytosis. Protein kinase C phosphorylates SNAP-25 at Ser187, however the physiological significance of this phosphorylation event in brain function remains unclear. We found that SNAP-25 phosphorylation increased rapidly in the mouse brain following cold-water restraint stress. Both basal and stress-induced phosphorylation of SNAP-25 were high in stress-related brain regions and the extent of phosphorylation increased with increasing amounts of stress. Intravenous administration of adrenaline increased SNAP-25 phosphorylation, although stress-induced phosphorylation was still observed in adrenalectomized mice. These results indicate that SNAP-25 phosphorylation is regulated in a stress-dependent manner through both central and peripheral mechanisms. We also investigated SNAP-25 dephosphorylation, and found that protein phosphatase 2A participates in SNAP-25 dephosphorylation through Ca2+-dependent and independent mechanisms.

  • Research Products

    (4 results)

All 2014 2013 2012

All Journal Article (2 results) (of which Peer Reviewed: 2 results) Presentation (2 results)

  • [Journal Article] Stress-induced phosphorylation of SNAP-252014

    • Author(s)
      Yamamori S, Sugaya D, Iida Y, Kokubo H, Itakura M, Suzuki E, Kataoka M, Miyaoka H, Takahashi M.
    • Journal Title

      Neuroscience Letters 2014

      Volume: 561 Pages: 182-187

    • DOI

      10.1016/j

    • Peer Reviewed
  • [Journal Article] Protein phosphatase 2A dephosphorylates SNAP-25 through two distinct mechanisms in mouse brain synaptosomes2013

    • Author(s)
      Iida Y, Yamamori S, Itakura M, Miyaoka H, Takahashi M.
    • Journal Title

      Neurosci Reserch 2013

      Volume: 75 Pages: 184-189

    • DOI

      10.1016/j

    • Peer Reviewed
  • [Presentation] PKC-dependent phosphorylation of SNAP-25 is essential for the positive regulation of dopamine release in mouse brain2013

    • Author(s)
      Yamamori S, Kanno S, Takahashi M.
    • Organizer
      北米神経科学会
    • Place of Presentation
      アメリカSan Diego
    • Year and Date
      2013-11-11
  • [Presentation] Stress induced phosphorylation of Snap-252012

    • Author(s)
      Yamamori S, Sugaya D, Iida Y, Kokubo H, Itakura M, Suzuki E, Kataoka M, Miyaoka H, Takahashi M.
    • Organizer
      北米神経化学会
    • Place of Presentation
      アメリカNew Orleans
    • Year and Date
      2012-10-17

URL: 

Published: 2015-06-25  

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