2014 Fiscal Year Final Research Report
Analysis of male germ cell specific GPI-anchored proteins using gene-manipulated mice
| Project/Area Number |
24700430
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory animal science
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 雄性生殖細胞 / GPIアンカータンパク質 / 精子受精能 / 遺伝子組換えマウス |
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| Outline of Final Research Achievements |
Germ cells are the only cells that can carry genetic information on to the next generation. I focused on the male germ cell specific glycosylphosphatidylinositol (GPI)-anchored protein complexes, TEX101 and LY6K, and studied their physiological functions using gene-manipulated mice.
I showed that these GPI-anchored protein complexes are required for sperm migrating ability into the oviduct and subsequent male fertility (PNAS. 2013, BOR. 2014). TEX101 and LY6K protein complexes interacted with the sperm membrane protein ADAM3 in mouse testes. However, spermatozoa from LY6K-deficient mice had no aberrance in ADAM3. Thus, the no effect on ADAM3 in LY6K-deficient mice is indicative of an as yet undefined pathway in the mouse.
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| Free Research Field |
生殖生物学、発生工学、実験動物学
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