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2013 Fiscal Year Final Research Report

Establishment of safe and efficient differentiation system using automatic removal vector

Research Project

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Project/Area Number 24700474
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Biomedical engineering/Biological material science
Research InstitutionUniversity of Tsukuba

Principal Investigator

NISHIMURA Ken  筑波大学, 医学医療系, 助教 (80500610)

Project Period (FY) 2012-04-01 – 2014-03-31
Keywords分化 / 多能性幹細胞 / 残存未分化細胞 / SeVdpベクター / miRNA
Research Abstract

When we induce differentiated cells from pluripotent stem cells, the risks of tumor formation by remained undifferentiated cells must be concerned. To reduce such a risk, we tried to establish a system for elimination of undifferentiated cells in the differentiated tissue using our SeVdp vector system. The SeVdp vector which has the target sequence of neuron-specific miRNA was infected to mouse iPS cells, and then they were differentiated to neuron. As a result, the vector were automatically removed from neuron while it was persistently infected in undifferentiated cells. But when we tried to select differentiated cells using negative selection gene from the vector, not only undifferentiated cells but also differentiated cells were killed by the treatment. We plan to investigate other method of differentiation and negative selection to selectively eliminate the undifferentiated cells.

  • Research Products

    (12 results)

All 2014 2013 2012

All Journal Article (5 results) (of which Peer Reviewed: 5 results) Presentation (7 results)

  • [Journal Article] Heterogeneous Nuclear Ribonucleoprotein R Cooperates with Mediator to Facilitate Transcription Reinitiation on the c-Fos Gene2013

    • Author(s)
      A. Fukuda, M. Shimada, T. Nakadai, K. Nishimura, K. Hisatake
    • Journal Title

      PLOS ONE

      Volume: Vol. 8

    • DOI

      10.1371/journal.pone.0072496

    • Peer Reviewed
  • [Journal Article] GPAT2, a mitochondrial outer membrane protein, in piRNA biogenesis in germline stem cells2013

    • Author(s)
      Y. Shiromoto, S. Kuramochi-Miyagawa, A. Daiba, S. Chuma, A. Katanaya, A. Katsumata, K. Nishimura, M. Ohtaka, M. Nakanishi, T. Nakamura, K. Yoshinaga, N. Asada, S. Nakamura, T. Yasunaga, K. Kojima-Kita, D. Itou, T. Kimura, T. Nakano
    • Journal Title

      RNA

      Volume: Vol. 19 Pages: 803-810

    • Peer Reviewed
  • [Journal Article] Expansion of Functional Human Mucosal-Associated Invariant T Cells via Reprogramming to Pluripotency and Redifferentiation2013

    • Author(s)
      H. Wakao, K. Yoshikiyo, U. Koshimizu, T. Furukawa, K. Enomoto, T. Matsunaga, T. Tanaka, Y. Yasutomi, T. Yamada, H. Minakami, J. Tanaka, A. Oda, T. Sasaki, R. Wakao, O. Lantz, T. Udagawa, Y. Sekiya, K. Higuchi, N. Harada, K. Nishimura, M. Ohtaka, M. Nakanishi, H. Fujita
    • Journal Title

      Cell Stem Cells

      Volume: Vol. 12 Pages: 546-558

    • Peer Reviewed
  • [Journal Article] Generation of rejuvenated antigen-specific T cells by reprogramming to pluripotency and redifferentiation2013

    • Author(s)
      T. Nishimura, S. Kaneko, A. Kawana-Tachikawa, Y. Tajima, H. Goto, D. Zhu, K. Nakayama-Hosoya, S. Iriguchi, Y. Uemura, T. Shimizu, N. Takayama, D. Yamada, K. Nishimura, M. Ohtaka, N. Watanabe, S. Takahashi, A. Iwamoto, H. Koseki, M. Nakanishi, K. Eto, H. Nakauchi
    • Journal Title

      Cell Stem Cells

      Volume: Vol. 12 Pages: 114-126

    • Peer Reviewed
  • [Journal Article] Podocalyxin is a Glycoprotein Ligand of the Human Pluripotent Stem Cell-Specific Probe rBC2LCN2013

    • Author(s)
      H. Tateno, A. Matsushima, K. Hiemori, Y. Onuma, Y. Ito, K. Hasehira, K. Nishimura, M. Ohtaka, S. Takayasu, M. Nakanishi, Y. Ikehara, M. Nakanishi, K. Ohnuma, T. Chan, M. Toyoda, H. Akutsu, A. Umezawa, M. Asashima, J. Hirabayashi
    • Journal Title

      Stem Cells Trans. Med.

      Volume: Vol. 2 Pages: 265-273

    • Peer Reviewed
  • [Presentation] 初期化が途中で停止した細胞を用いたiPS細胞誘導過程の解析2014

    • Author(s)
      西村健
    • Organizer
      第13回日本再生医療学会総会
    • Place of Presentation
      京都国際会議場、京都
    • Year and Date
      2014-03-05
  • [Presentation] Klf4遺伝子発現量調節による初期化が途中で停止した細胞の誘導2013

    • Author(s)
      西村健
    • Organizer
      第36回日本分子生物学会年会
    • Place of Presentation
      神戸国際展示場、神戸
    • Year and Date
      2013-12-03
  • [Presentation] Manipulation of Klf4 expression produces snap shots of iPSC induction2013

    • Author(s)
      K. Nishimura
    • Organizer
      CSHA/ISSCR Joint Meeting on Stem Cells in Science and Medicine
    • Place of Presentation
      Suzhou Dushu Lake Conference Center、Suzhou、China
    • Year and Date
      2013-10-16
  • [Presentation] Taking snap shots during iPSC production by SeVdp vectors2013

    • Author(s)
      K. Nishimura
    • Organizer
      International Society of Stem Cell Research 11^<th> Annual Meeting
    • Place of Presentation
      The Boston Convention Center、Boston、USA
    • Year and Date
      2013-06-14
  • [Presentation] SeVdpベクターを用いたiPS細胞の品質調節メカニズムの解析2013

    • Author(s)
      西村健
    • Organizer
      第12回日本再生医療学会総会
    • Place of Presentation
      パシフィコ横浜、横浜
    • Year and Date
      2013-03-22
  • [Presentation] Application of Cytoplasmic RNA vector Developed from Noncytopathic RNA Viruses to Stem Cell Engineering2013

    • Author(s)
      K. Nishimura
    • Organizer
      The 2nd Meeting on RNA and Biofunctions-Asia Study
    • Place of Presentation
      ホテルレオパレス福岡、福岡
    • Year and Date
      2013-01-09
  • [Presentation] QUALITY CONTROL OF IPS CELLS BY UNIQUE GENE TRANSFER SYSTEM2012

    • Author(s)
      K. Nishimura
    • Organizer
      International Society of Stem Cell Research 10^<th> Annual Meeting
    • Place of Presentation
      パシフィコ横浜、横浜
    • Year and Date
      2012-06-14

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Published: 2015-06-25  

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