2013 Fiscal Year Annual Research Report
疾患早期発見を目指す金ナノ粒子－表面増強ラマン散乱法による細胞内炎症反応の検出
| Project/Area Number |
24700481
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| Research Institution | Osaka University |
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Principal Investigator |
ピスワン ダーコン 大阪大学, 免疫学フロンティア研究センター, 特任研究員(常勤) (40622147)
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| Keywords | 金ナノ粒子 / SERS / 金ナノ粒子 / ICAM-1 |
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| Research Abstract |
This research is to investigate the use of surface enhance Raman scattering (SERS)-dynamic gold nanoparticles for rapid monitoring of inflammatory responses that can indicate the early stage of diseases. Gold nanorods(GNRs) completely combined with Raman probe (4-mercaptobenzoic acid) and then attached with intercellular adhesion molecule-1 antibody (anti-ICAM-1).The prepared particles were used as a tool to detect the expression of ICAM-1 on the surface of inflamed macrophages. This technique could significantly detect the expression of ICAM-1 on the surface of macrophages stimulated by lipopolysaccharides for 1 hour. However, the detection of ICAM-1 using ELISA assay and fluorescent techniques needed a stimulation period for 5 hours. This work was submitted for consideration to publish in ISI journal. The investigation of the effect of GNRs on inflammatory cytokine response was also performed to confirm that the GNRS have no effect to induce inflammation in macrophages. This result was published in Particle and Particle System Characterization. SERS signal detection in live macrophages using labelled free GNRs was performed and published in RSC Advances. GNRs combined with Raman spectroscopy could be used as a detection tool to detect the expression of ICAM-1 molecules on the cell surface at the early stage. This technique would be developed further to increase more efficiency of detection and to expand the detection technique in vivo.
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