2013 Fiscal Year Final Research Report
Early detection of disease using surface enhance Raman scattering (SERS)-dynamic gold nanoparticle to detect inflammatory response in cells
| Project/Area Number |
24700481
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biomedical engineering/Biological material science
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| Research Institution | Osaka University |
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Principal Investigator |
PISSUWAN Dakrong 大阪大学, 免疫学フロンティア研究センター, 特任研究員(常勤) (40622147)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 金ナノ粒子 / SERS / 炎症反応 / ICAM-1 |
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| Research Abstract |
The investigation of the use of surface enhanced Raman scattering (SERS) gold nanoparticles for rapid monitoring of inflammatory responses at the early stage of diseases. Gold nanorods (GNRs) combined with Raman probes (4-mercaptobenzoic acid) are conjugated with intercellular adhesion molecule-1 antibody (anti-ICAM-1). The prepared particles could be used as a SERS probe to detect the expression of ICAM-1 in macrophages treated lipopolysaccharide for 1 h which is faster than using ELISA or fluorescent labeling techniques.
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