The development of reliable biomarker measurement systems considered for human cancer tissue heterogeneity.

2015 Fiscal Year Final Research Report

• Project/Area Number: 24700501
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical systems
• Research Institution: Tokyo Medical University
• Principal Investigator: Nagai Takeshi 東京医科大学, 医学部, 兼任助教 (60384989)
• Research Collaborator: Oshiro Hisashi 自治医科大学, 医学部, 教授 (60381513)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Keywords: 仮想組織マイクロアレイ / 病理診断学 / Ki-67 index / Apoptosis index / digital microscopy / Bland-Altman plot 分析 / systematic bias / ヒト癌組織の不均一性

Outline of Final Research Achievements

Digital microscopy-assisted, computerized image analysis enabled us to easily quantify histopathological heterogeneity in human cancer tissues, leading to a high level of quantitative and reproducible capability for biomarker measurement results. Our study suggests that the measurement results for certain biomarkers obtained from tissue microarray-based samples can be susceptible to systematic bias, and the lack of correlation between biomarkers cannot be avoided as it is in whole mount tissue preparations. Virtual tissue microarray in combination with Bland-Altman plot analysis can be useful for identifying a systematic bias, and this will help to establish a better sampling strategy for reliable biomarker measurements considered for human cancer tissue heterogeneity.

Free Research Field

病理学