2013 Fiscal Year Final Research Report
Proteomic analysis of nitrated proteins in the skeletal muscle after physical exercise.
| Project/Area Number |
24700761
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied health science
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| Research Institution | Juntendo University |
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Principal Investigator |
UDA Munehiro 順天堂大学, 医学(系)研究科(研究院), 研究員 (80549262)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | ニトロトリプトファン / 高強度間欠性走運動 / 細胞内シグナル伝達タンパク質 / プロテオーム解析 |
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| Research Abstract |
We identified nitrotyptophan-containing proteins from adult rat skeletal muscle under physiological condition. The degree of typtophan nitration in the skeletal muscle were not changed by high intensity intermittent running. Thus, nitrotyptophan-containing proteins under physiological condition may not function as a scavenger of reactive oxygen and nitrogen species. In addition, the typtophan nitration of alpha-skeletal muscle actin, which is detected at approximately 60 kDa, and ERK that is involved in the signal transduction in cells were not changed by high intensity intermittent running.
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