2014 Fiscal Year Final Research Report
Quantification of the contribution of each pathway in N2O production from nitrifying bacteria by using a dual-labeled tracer technique
| Project/Area Number |
24710012
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Environmental dynamic analysis
|
|---|
| Research Institution | Chuo University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Keywords | 安定同位体トレーサー / アンモニア酸化細菌 / 亜酸化窒素 / 硝化 / 脱窒 / 窒素循環 / 温室効果ガス / ガスクロマトグラフィー質量分析計 |
|---|
| Outline of Final Research Achievements |
Ammonia-oxidizing bacteria (AOB) have been shown to produce nitrous oxide (N2O) from hydroxylamine (NH2OH) during ammonia oxidization and/or from nitrite (NO2-) during nitrifier denitrification under aerobic condition. The aim of this study is to determine which mechanisms are responsible for producing N2O by AOB species using a 15N and 18O tracer technique. (1) The fragmentation and the scrambling ratio of N2O were determined by GC/MS. (2) Cell suspension of Nitrosomonas strains formed hybrid N2O from NH2OH and NO2-. The dominant pathway in N2O production differed among Nitrosomonas strains under a condition, where they were allowed to grow optimally.
|
| Free Research Field |
微生物生態学
|
