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2013 Fiscal Year Final Research Report

Real-time monitoring of chemical transport on cell membrane using nano-SECM

Research Project

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Project/Area Number 24710140
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Microdevices/Nanodevices
Research InstitutionTohoku University

Principal Investigator

TAKAHASHI YASUFUMI  東北大学, 原子分子材料科学高等研究機構, 助教 (90624841)

Project Period (FY) 2012-04-01 – 2014-03-31
Keywords走査プローブ顕微鏡 / マイクロ・ナノデンバイス / バイオ関連機器 / 細胞・組織 / 分析科学
Research Abstract

Chemical transport on the cell membrane is important issue for developement of drug delivery system and improvement of gene transfection. In this study, the dynamic cell surface fluctuation was detected or visualised by using nano electrode. The electrochemical measurement is effective for evaluation of mass transfer on liquid/solid interface. I developed fabrication method of nanoelectrode and nanopositioning system for quantitative analysis and time-lapse imaging of mass transfer on cell membrane.

  • Research Products

    (39 results)

All 2014 2013 2012 Other

All Journal Article (17 results) (of which Peer Reviewed: 17 results) Presentation (19 results) (of which Invited: 3 results) Book (2 results) Remarks (1 results)

  • [Journal Article] Isolation and quantification of messenger RNA from tissue models by using a double-barrel carbon probe2014

    • Author(s)
      Nashimoto, Y., Takahashi, Y., Takano, R., Miyashita, K., Yamada, S., Ino, K., Shiku, H., Matsue, T.
    • Journal Title

      Anal. Bioanal. Chem

      Volume: 406(1) Pages: 275-282

    • Peer Reviewed
  • [Journal Article] SU-8-based Flexible Amperometric Device with IDA Electrodes to Regenerate Redox Species in Small Spaces2014

    • Author(s)
      Kanno, Y., Goto, T., Ino, K., Inoue, K. Y., Takahashi, Y., Shiku, H., Matsue, T.
    • Journal Title

      Anal. Sci

      Volume: 30(2) Pages: 305-309

    • Peer Reviewed
  • [Journal Article] Densified Electrochemical Sensors Based on Local Redox Cycling between Vertically Separated Electrodes in Substrate Generation/Chip Collection and Extended Feedback Modes2014

    • Author(s)
      Ino, K., Kanno, Y., Nishijo, T., Komaki, H., Yamada, Y., Yoshida, S., Takahashi, Y., Shiku, H., Matsue, T.
    • Journal Title

      Anal. Chem

      Volume: 86(8) Pages: 4016-4023

    • Peer Reviewed
  • [Journal Article] Droplet array on local redox cycling-based electrochemical (LRC-EC) chip device2014

    • Author(s)
      Ino, K., Goto, T., Kanno, Y., Inoue, K. Y., Takahashi, Y., Shiku, H., Matsue, T.
    • Journal Title

      Lab Chip

      Volume: 14(4) Pages: 787-794

    • Peer Reviewed
  • [Journal Article] Electrochemical Nanoprobes for Single-Cell Analysis2014

    • Author(s)
      Actis, P., Tokar, S., Clausmeyer, J., Babakinejad, B., Mikhaleva, S., Cornut, R., Takahashi, Y., Cordoba, A. L., Novak, P., Shevchuck, A. I., Dougan, J. A., Kazarian, S. G., Gorelkin, P. V., Erofeev, A. S., Yaminsky, I. V., Unwin, P. R., Schuhmann, W., Klenerman, D., Rusakov, D. A., Sviderskaya, E. V., Korchev, Y. E.
    • Journal Title

      Acs Nano

      Volume: 8(1) Pages: 875-884

    • Peer Reviewed
  • [Journal Article] Electrodeposition of alginate gels for construction of vascular-like structures2013

    • Author(s)
      Ozawa, F., Ino, K., Takahashi, Y., Shiku, H., Matsue, T.
    • Journal Title

      J. Biosci. Bioeng

      Volume: 115(4) Pages: 459-461

    • Peer Reviewed
  • [Journal Article] Alginate gel microwell arrays using electrodeposition for three-dimensional cell culture2013

    • Author(s)
      Ozawa, F., Ino, K., Arai, T., Ramon-Azcon, J., Takahashi, Y., Shiku, H., Matsue, T.
    • Journal Title

      Lab Chip

      Volume: 13(15) Pages: 3128-3135

    • Peer Reviewed
  • [Journal Article] Fabrication, Characterization, and Functionalization of Dual Carbon Electrodes as Probes for Scanning Electrochemical Microscopy (SECM)2013

    • Author(s)
      McKelvey, K., Nadappuram, B. P., Actis, P., Takahashi, Y., Korchev, Y. E., Matsue, T., Robinson, C., Unwin, P. R.
    • Journal Title

      Anal. Chem

      Volume: 85(15) Pages: 7519-7526

    • Peer Reviewed
  • [Journal Article] Evaluation of the differentiation status of single embryonic stem cells using scanning electrochemical microscopy2013

    • Author(s)
      Matsumae, Y., Arai, T., Takahashi, Y., Ino, K., Shiku, H., Matsue, T.
    • Journal Title

      Chem. Commun

      Volume: 49(58) Pages: 6498-6500

    • Peer Reviewed
  • [Journal Article] Carbon-Ag/AgCl Probes for Detection of Cell Activity in Droplets2013

    • Author(s)
      Ino, K., Ono, K., Arai, T., Takahashi, Y., Shiku, H., Matsue, T.
    • Journal Title

      Anal. Chem

      Volume: 85(8) Pages: 3832-3835

    • Peer Reviewed
  • [Journal Article] Electrochemical Device with Interdigitated Ring Array Electrodes for Investigating the Relationship between Cardiomyocyte Differentiation from Embryonic Stem Cells and Alkaline Phosphatase Activity2013

    • Author(s)
      Ino, K., Nishijo, T., Kanno, Y., Ozawa, F., Arai, T., Takahashi, Y., Shiku, H., Matsue, T.
    • Journal Title

      Electrochemistry

      Volume: 81(9) Pages: 682-687

    • Peer Reviewed
  • [Journal Article] Development of Carbon Nano-Heater : Stimulation of Sensory Neurons for Functional Study of Heat Sensitive Channels (vol 104, pg 519a, 2013)2013

    • Author(s)
      Babakinejad, B., Yasufumi, T., Korchev, Y., Actis, P.
    • Journal Title

      Biophys. J

      Volume: 104(8) Pages: 1834-1834

    • Peer Reviewed
  • [Journal Article] Local Delivery of Molecules from a Nanopipette for Quantitative Receptor Mapping on Live Cells2013

    • Author(s)
      Babakinejad, B., Jonsson, P., Cordoba, L., Actis, P., Novak, P., Takahashi, Y., Shevchuk, A., Anand, U., Anand, P., Drews, A., Ferrer-Montiel, A., Klenerman, D., Korchev, Y. E.
    • Journal Title

      Anal. Chem

      Volume: 85(19) Pages: 9333-9342

    • Peer Reviewed
  • [Journal Article] Topographical and electrochemical nanoscale imaging of living cells using voltage-switching mode scanning electrochemical microscopy2012

    • Author(s)
      Takahashi, Y., Shevchuk, A. I., Novak, P., Babakinejad, B., Macpherson, J., Unwin, P. R., Shiku, H., Gorelik, J., Klenerman, D., Korchev, Y. E., Matsue, T.
    • Journal Title

      Proc. Natl. Acad. Sci. U. S. A

      Volume: 109(29) Pages: 11540-11545

    • Peer Reviewed
  • [Journal Article] Local Redox-Cycling-Based Electrochemical Chip Device with Deep Microwells for Evaluation of Embryoid Bodies2012

    • Author(s)
      Ino, K., Nishijo, T., Arai, T., Kanno, Y., Takahashi, Y., Shiku, H., Matsue, T.
    • Journal Title

      Angew. Chem. Int. Ed

      Volume: 51(27) Pages: 6648-6652

    • Peer Reviewed
  • [Journal Article] Electrochemical detection for dynamic analyses of a redox component in droplets using a local redox cycling-based electrochemical (LRC-EC) chip device2012

    • Author(s)
      Ino, K., Kanno, Y., Nishijo, T., Goto, T., Arai, T., Takahashi, Y., Shiku, H., Matsue, T.
    • Journal Title

      Chem. Commun

      Volume: 48(68) Pages: 8505-8507

    • Peer Reviewed
  • [Journal Article] Novel Electrochemical Methodology for Activity Estimation of Alkaline Phosphatase Based on Solubility Difference2012

    • Author(s)
      Ino, K., Kanno, Y., Arai, T., Inoue, K. Y., Takahashi, Y., Shiku, H., Matsue, T.
    • Journal Title

      Anal. Chem

      Volume: 84(18) Pages: 7593-7598

    • Peer Reviewed
  • [Presentation] 界面イオン伝導顕微鏡による多結晶電極表面の結晶構造とLi挿入脱離の相関性の検討2014

    • Author(s)
      熊谷明哉, 高橋康史, 猪又宏貴, 白木将, 山本邦子, 春田正和, 伊野浩介, 珠玖仁, 一杉太郎, 末永智一
    • Organizer
      電気化学会第81回大会
    • Place of Presentation
      関西大学
    • Year and Date
      2014-03-31
  • [Presentation] Development of Voltage Switching Mode Scanning Electrochemical Microscopy for Nanoscale Electrochemical Imaging2014

    • Author(s)
      Takahashi, Y., Matsumae, Y., Ino, K., Shiku, H., Korchev, Y., Matsue, T.
    • Organizer
      14th Topical Meeting of the International Society of Electrochemistry in Nanjing
    • Place of Presentation
      China
    • Year and Date
      2014-03-30
    • Invited
  • [Presentation] 界面イオン伝導顕微鏡の創製とLiイオン電池電極材料への応用2014

    • Author(s)
      熊谷明哉, 高橋康史, 猪又宏貴, 棟方裕一, 伊野浩介, 珠玖仁, 金村聖志, 末永智一
    • Organizer
      第61回応用物理学会春季学術講演会
    • Place of Presentation
      青山学院大学
    • Year and Date
      2014-03-19
  • [Presentation] 走査型イオンコンダクタンス顕微鏡を用いた海馬の形状イメージ2013

    • Author(s)
      高橋康史, 松前義治, 伊野浩介, 珠玖仁, 末永智一
    • Organizer
      バイオ系SPM研究会
    • Place of Presentation
      新潟
    • Year and Date
      2013-12-09
  • [Presentation] Nanoscale Imaging of Living Cells using Nano-Scanning Electrochemical Microscopy2013

    • Author(s)
      Takahashi, Y., Matsumae, Y., Ino, K., Shiku, H., Korchev, Y., Matsue, T.
    • Organizer
      ICFD2013 (Special Session of Tenth International Conference on Fluid Dynamics)
    • Place of Presentation
      Sendai
    • Year and Date
      2013-11-25
  • [Presentation] 走査型電気化学・走査型イオンコンダクタンス顕微鏡を用いた生体イメージング2013

    • Author(s)
      高橋康史, 松前義治, 伊野浩介, 珠玖仁, 末永智一
    • Organizer
      公益社団法人日本顕微鏡学会第57回シンポジウム
    • Place of Presentation
      名古屋
    • Year and Date
      2013-11-16
    • Invited
  • [Presentation] 界面イオン伝導顕微鏡による電極材料表面におけるLi挿入脱離の可視化2013

    • Author(s)
      熊谷明哉, 高橋康史, 棟方裕一, 猪又宏貴, 伊野浩介, 珠玖仁, 金村聖志, 末永智一
    • Organizer
      第54回電池討論会
    • Place of Presentation
      大阪
    • Year and Date
      2013-10-08
  • [Presentation] 界面イオン伝導顕微鏡によるLiFePO4電極材料表面のその場観察2013

    • Author(s)
      高橋康史, 熊谷明哉, 棟方裕一, 伊野浩介, 珠玖仁, 金村聖志, 末永智一
    • Organizer
      2013年電気化学秋季大会
    • Place of Presentation
      東京
    • Year and Date
      2013-09-27
  • [Presentation] Topography Imaging of Hippocampus Spine using Scanning Ion Conductance Microscopy2013

    • Author(s)
      Takahashi, Y., Matsumae, Y., Ino, K., Shiku, H., Korchev, Y., Matsue, T.
    • Organizer
      XXIII International Symposium on Morphological Sciences
    • Place of Presentation
      Niigata
    • Year and Date
      2013-09-20
  • [Presentation] In-situ characterization and mapping of lithium-ion transport at the electrolyte/cathode interface by high resolution ion-conductance microscopy2013

    • Author(s)
      Kumatani, A, Takahashi, Y., H. Munakata, H., Inomata, H., Ino, K., Shiku, H., Kanamura, K., Matsue, T.
    • Organizer
      EMRS fall meeting 2013
    • Place of Presentation
      Warsaw, Poland
    • Year and Date
      2013-09-17
  • [Presentation] Topographical and Electrochemical Nanoscale Imaging of Living Cells using Nano-Scanning Electrochemical Microscopy2013

    • Author(s)
      Takahashi, Y., Matsumae, Y., Ino, K., Shiku, H., Korchev, Y., Matsue, T.
    • Organizer
      The Fourteenth International Symposium on Electroanalytical Chemistry
    • Place of Presentation
      Changchun, China
    • Year and Date
      2013-08-20
    • Invited
  • [Presentation] Evaluation of Differentiation State of an Embryonic Stem Cell using Scanning Electrochemical Microscopy2013

    • Author(s)
      Takahashi, Y., Matsumae, Y., Ino, K., Shiku, H., Matsue, T.
    • Organizer
      223st ECS Meeting
    • Place of Presentation
      Toronto, Canada
    • Year and Date
      2013-05-12
  • [Presentation] ナノピペットを利用したLiFePO_4の局所的充放電特性評価2013

    • Author(s)
      高橋康史,伊藤小町,松前義治,棟方裕一,珠玖仁,伊野浩介,金村聖志,末永智一
    • Organizer
      電気化学会第80回大会
    • Place of Presentation
      仙台
    • Year and Date
      2013-03-28
  • [Presentation] 走査型電気化学・イオンダクタンス顕微鏡を用いたバイオイメージング2012

    • Author(s)
      高橋康史, 松前義治, 伊野浩介, 珠玖仁, 末永智一
    • Organizer
      生体SPM研究部会
    • Place of Presentation
      新潟
    • Year and Date
      2012-12-09
  • [Presentation] Electrochemical and Topographical Imaging of Living Cell using SECM-SICM2012

    • Author(s)
      Takahashi, Y., Matsumae, Y., Ino, K., Shiku, H., Korchev, Y., Matsue, T.
    • Organizer
      3rd Kanazawa Bio-AFM Workshop
    • Place of Presentation
      Kanazawa
    • Year and Date
      2012-11-05
  • [Presentation] Development of Voltage Switching Mode Scanning Electrochemical Microscopy for Topographical and Electrochemical Nanoscale Imaging of Living Cells2012

    • Author(s)
      Takahashi, Y., Matsumae, Y., Ino, K., Shiku, H., Korchev, Y., Matsue, T.
    • Organizer
      222st ECS Meeting
    • Place of Presentation
      Hawaii, USA
    • Year and Date
      2012-10-09
  • [Presentation] ナノ電気化学顕微鏡による膜タンパク質のイメージング2012

    • Author(s)
      高橋康史, 松前義治, 伊野浩介, 珠玖仁, 末永智一
    • Organizer
      日本分析化学会第61年会
    • Place of Presentation
      金沢
    • Year and Date
      2012-09-20
  • [Presentation] High-resolution bioelectrochemical imaging with SECM-SICM hybrid system2012

    • Author(s)
      Takahashi, Y., Matsumae, Y., Ino, K., Shiku, H., Korchev, Y., Matsue, T.
    • Organizer
      International Association of Colloid and Interface Scientists Conference 2012
    • Place of Presentation
      Sendai
    • Year and Date
      2012-05-16
  • [Presentation] Characterize Cell Function using SECMSICM2012

    • Author(s)
      Takahashi, Y., Matsumae, Y., Ino, K., Shiku, H., Korchev, Y., Matsue, T.
    • Organizer
      221st ECS Meeting
    • Place of Presentation
      Seattle, USA
    • Year and Date
      2012-05-07
  • [Book] マイクロナノ電極システムを用いたバイオセンシングとバイオイメージング, 表面科学2013

    • Author(s)
      高橋康史, 珠玖仁, 末永智一
    • Total Pages
      488-493
  • [Book] 「使える解析テクニック!」シリーズ, マイクロ電極(3)応用計測事例:電気化学顕微鏡(SECM)-走査型イオンコンダクタンス顕微鏡(SICM)複合システムの開発2012

    • Author(s)
      高橋康史, 珠玖仁, 末永智一
    • Total Pages
      271-275
    • Publisher
      Electrochemistry
  • [Remarks]

    • URL

      http://www.che.tohoku.ac.jp/~bioinfo/

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Published: 2015-06-25  

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