Investigation into the mechanism of transcription-coupled chromatin regulation

2013 Fiscal Year Final Research Report

• Project/Area Number: 24710229
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: System genome science
• Research Institution: Shimane University
• Principal Investigator: KATO Hiroaki 島根大学, 医学部, 助教 (40548418)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: エピゲノム / エピゲノム制御 / 転写 / ヒストン / ヌクレオソーム / Spt6 / Iws1 / RNAポリメラーゼII

Research Abstract

In this study, aiming to understand the molecular mechanism of transcription-coupled chromatin regulation, we obtained following observations. 1) A point mutation of the Spt6-interacting transcription machinery RNA polymerase II gradually derepressed pericentromeric silencing that had been normal. 2) Another Spt6-interacting protein Iws1 contributes the maintenance of histone occupancy in the transcribed regions as in the case of Spt6, and represses transcription-coupled shifting of nucleosomes. These data suggest that, RNA polymerase, in conjunction with Spt6 and Iws1, need to transcribe nucleosome templates carefully, in order to maintain epigenetic memory in the transcribed region.

Research Products

• [Journal Article] Mediator directs co-transcriptional heterochromatin assembly by RNA interference- dependent and -independent path- ways (2013)
  • Author(s): Oya E, Kato H, Chikashige Y, Tsutsumi C, Hiraoka Y, Murakami Y
  • Journal Title: PLoS Genetics Volume: vol.9 No.8 Pages: 1003677
  • DOI: 10.1371/journal.pgen.1003677
  • Peer Reviewed
• [Journal Article] Spt6 prevents transcription- coupled loss of posttranslationally modified histone H3 (2013)
  • Author(s): Kato H, Okazaki K, Iida T, Nakayama J, Murakami Y, Urano T
  • Journal Title: Scientific Reports Volume: vol.3 Pages: 2186
  • DOI: 10.1038/srep02186
  • Peer Reviewed
• [Journal Article] Spt6 : Two fundamentally distinct functions in the regulation of histone modification (2013)
  • Author(s): Kato H, Okazaki K, Urano T
  • Journal Title: Epigenetics Volume: vol.8 No.12 Pages: 1249-1253
  • DOI: 10.4161/epi.26487
  • Peer Reviewed
• [Journal Article] The transcription factors Atf1 and Pcr1 are essential for transcriptional induction of the extracellular maltase Agl1 in fission yeast (2013)
  • Author(s): Kato H, Kira S, Kawamukai M
  • Journal Title: PLoS One Volume: vol.8 No.11 Pages: 80572
  • DOI: 10.1371/journal.pone.0080572
  • Peer Reviewed
• [Presentation] Spt6は既存のヒストンH3の転写と共役した消失を防ぎ遺伝子座特異的修飾を維持する (2013)
  • Author(s): 加藤太陽
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸市・ポートピアホテル
  • Year and Date: 2013-12-04
• [Presentation] Spt6 prevents transcription- coupled loss of posttranslationally modified histone H3 (2013)
  • Author(s): Kato H
  • Organizer: Message from Yeast to Epigenetics
  • Place of Presentation: 福井県あわら市・グランディア芳泉
  • Year and Date: 2013-09-02
• [Remarks] アウトリーチ活動:出張講義講師,第三回 理科クラブ(主催者:飯南町教育委員会)ヒト培養細胞から抽出したDNAの観察,PCRで増幅したDNAの観察,および蛍光タンパク質(GFPとmCherry)の観察(2013年8月7日,島根 県飯南町・飯南高校)
• [Remarks]
  • URL: http://www.med.shimane-u.ac.jp/biochem2/index.html