2013 Fiscal Year Final Research Report

Study of nucleic acids detecting probe which recognaize 3'-end sequence of RNA by formation of boronic acid ester

Research Project

Project/Area Number	24710264
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	Chemical biology
Research Institution	Tokyo Institute of Technology (2013) Kanagawa University (2012)
Principal Investigator	OKAMOTO Itaru 東京工業大学, 生命理工学研究科, 東工大特別研究員 (40460133)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	RNA検出 / ボロン酸 / 蛍光 / 核酸
Research Abstract	The aim of this study is to develop new RNA detecting probe which consisting of a DNA strand with a fluorescing group and a phenyl boronic acid residue. The DNA strand of the probe binds to a 3'-end sequence of a target RNA to form a double helical structure, then the boronic acid residue of the probe and a diol group at the 3'- end of target RNA form boronic esters which results increasement of fluorescence intensity by cancelling photo-induced electron transfer (PET) between the fluorescent group and an amino group to which the phenyl boronic acid group attached. We successfully synthesized novel RNA detecting probe having a fluorescing group and phenyl boronic acid residue. When the probe formed a duplex with a target RNA, increasement of fluorescence intensity was observed. On the other hand, in the presence of a target DNA strand, increasement of fluorescence intensity was not observed.

(4 results)