2013 Fiscal Year Final Research Report
Development of sensitive and inexpensive antibody array chip
| Project/Area Number |
24760649
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biofunction/Bioprocess
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| Research Institution | Kyoto Institute of Technology |
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Principal Investigator |
KUMADA Yoichi 京都工芸繊維大学, 工芸科学研究科, 准教授 (70452373)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | PMMA-tag / 単鎖抗体 / 配向固定 |
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| Research Abstract |
In this study, we investigated to establish technologies for fabricatioin of antibody-based microarray chips immobilized with single-chain Fv antibodies (scFvs) by utilizing our original solid-phase refolding technology and conventional microarray spotting technology. Consequently, we found that our originally-isolated peptide tag specific to poly(methyl methacrylate) was significantly useful for efficient refolding and site-specific immobilization of scFv onto the surface of PMMA plate. Thus, we successfully established preparation method of scFv-immobilized PMMA plate and sensitive detection of antigen could be attained.
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