Development of culture method for evaluation of contractile capability of human skeletal muscle cells

2013 Fiscal Year Final Research Report

• Project/Area Number: 24760650
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Biofunction/Bioprocess
• Research Institution: Osaka University
• Principal Investigator: NAGAMORI Eiji 大阪大学, 工学(系)研究科(研究院), 講師 (70394898)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 骨格筋 / 筋芽細胞 / 繊維芽細胞 / 張力測定 / 細胞シート / 棲み分け / 自己組織化 / 遊走

Research Abstract

To enhance active tension capability of human skeletal muscle myotubes derived from human skeletal muscle myoblast population, addition of human skeletal muscle fibroblast cells to myoblast population was examined. Contrary to our expectation, significant improvement of contractile capability of human skeletal muscle myotubes was not achieved, which may be caused by spatial habitation of fibroblast cells in the myoblast tissue due to the differences in its behavior of migration and cell-cell connection. Fibroblast cells mixed in the myoblast tissue rapidly and linearly migrated in the vertical direction and distributed in upper position. Further studies investigating the habitation pattern of various cell types will shed light on controlling the spatial arrangement of the heterogeneous cell population and constructing complicated tissues.

Research Products

• [Journal Article] Spatial habitation of heterologous cells in a multi-layered myoblast sheet due to the differences in their behaviors of migration and cell-cell connection (2014)
  • Author(s): Nagamori, E., Oda, M., Nakamura, T., Shimizu, T., Okano, T., Kino-oka, M.
  • Journal Title: Cuurent Nanoscience Volume: 10 Pages: 173-178
• [Journal Article] Evaluation systems of generated forces of skeletal muscle cell-based bio-actuators (2013)
  • Author(s): Shimizu, K., Fujita, H., Nagamori, E.
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 115 Pages: 115-121
• [Journal Article] In vitro models for angiogenesis research : a review
  • Author(s): Ngo, T.X., Nagamori, E., Shimizu, T., Okano, T., Taya, M., Kino-oka, M.
  • Journal Title: International Journal of Tissue Regeneration Volume: (in press)
• [Presentation] 骨格筋由来筋芽細胞を用いた細胞アッセイ技術の開発と応用展開 (2014)
  • Author(s): 長森英二
  • Organizer: 2012年度日本動物細胞工学会奨励賞受賞講演会
  • Place of Presentation: 東京
  • Year and Date: 2014-02-10
• [Presentation] Spatial habitation of umbilical vein endothelial or fibroblast cells in multi-layered myoblast cell sheet (2012)
  • Author(s): Nagamori, E., Oda, M., Kino-oka, M.
  • Organizer: The 25th Annual and international meeting of the Japanese Association for Animal Cell Technology (JAACT2012)
  • Place of Presentation: 名古屋
  • Year and Date: 2012-11-30
• [Remarks] 長森 英二,平成24年度日本動物細胞工学会・研究奨励賞,論題:骨格筋由来筋芽細胞を用いた細胞アッセイ技術の開発と応用展開. (2013年7月19日)
• [Remarks] Best poster award in The 25th Annual and International Meeting of the Japanese Association for Animal Cell Technology (JAACT2012)論題:Spatial habitation of umbilical vein endothelial or fibroblast cells in multi-layered myoblast cell sheet. (2012年11月30日,発表者:長森 英二)