Elucidating the double fertilization mechanism controlled by sperm membrane structures

2014 Fiscal Year Final Research Report

• Project/Area Number: 24770062
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Morphology/Structure
• Research Institution: The University of Tokyo (2014); Waseda University (2012-2013)
• Principal Investigator: MORI Toshiyuki 東京大学, 理学(系)研究科(研究院), 特任研究員 (00462739)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 重複受精 / 配偶子 / 有性生殖 / 花粉 / 被子植物 / GCS1 / GEX2 / 膜タンパク質

Outline of Final Research Achievements

This study aimed to analyze gamete interaction during double fertilization specific to flowering plants using molecular cell biological strategies. Especially, this study focused on elucidating the molecular functions of transmembrane proteins identified in male gametes. When a proteomics analysis of Lilium generative cell membrane was performed based on the RNA sequencing data, a novel transmembrane protein named LGM1 was newly detected. LGM1 proved to be a small molecule, which possesses four transmembrane domains and conserved in algae, moss and lycophytes. As a result of molecular cytological analyses of LGM1, it was found that LGM1 is expressed specifically in male gametes.

Free Research Field

植物生理学