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2014 Fiscal Year Final Research Report

Monitoring temperature inside a single cell with a novel genetically encoded fluorescent temperature indicator

Research Project

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Project/Area Number 24770174
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Cell biology
Research InstitutionOsaka University

Principal Investigator

NAKANO MASAHIRO  大阪大学, 産業科学研究所, 助教 (30467617)

Project Period (FY) 2012-04-01 – 2015-03-31
Keywords温度 / 蛍光タンパク質
Outline of Final Research Achievements

Intracellular temperature is crucial role for many cellular processes such as gene expression and cellular metabolism. In this study, we developed a new genetically encoded fluorescent temperature indicator for monitoring temperature changing in a single living cell by combination a temperature sensitive fluorescent protein and a temperature less sensitive protein. To monitor the intracellular organelle specific temperature dynamics, we succeeded in expression the temperature indicator in mitochondria, nucleus, and plasma membrane. And also, we successfully monitored heat production in mitochondria of single cell with chemical stimulation.

Free Research Field

細胞生物学

URL: 

Published: 2016-06-03  

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