2015 Fiscal Year Final Research Report
Genome sequencing from a single nematode using whole genome amplification
| Project/Area Number |
24780044
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Plant pathology
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| Research Institution | University of Miyazaki |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | 寄生虫 / ゲノム / ゲノム増幅 |
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| Outline of Final Research Achievements |
Genome sequencing of uncultivable parasitic nematodes is difficult because of limited amount of DNA from isolated individuals. In this study we aimed to develop a novel and efficient method to sequence genome from a single nematode using whole genome amplification (WGA) and a Next generation sequencer (NGS). Based on evaluation of existing results of WGA samples we have designed three new methods of WGA-NGS. We evaluated the three methods in terms of amplification uniformity, SNP distribution and generation of chimera DNA. We found all the methods are useful for SNP detection, but they are different in chimeric DNA generation and a combination of multiple methods can help to achieve better quality of assembly.
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| Free Research Field |
寄生虫ゲノミクス
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