2015 Fiscal Year Final Research Report
Elucidation of signal transduction pathways for pellicle formation in wild yeast and its application to develop a novel method for preventing deleterious pellicle formation in wineries
| Project/Area Number |
24780069
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | University of Yamanashi |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Keywords | 野生酵母 / 産膜 / ワイン / FLO11 / FLO8 / 遺伝子破壊 |
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| Outline of Final Research Achievements |
In this study, we revealed that the glucose signal transduction pathway acting on FLO11 gene, which encodes a cell surface glycoprotein, is one of the signal transduction pathways for pellicle formation by a wild pellicle-forming strain of Saccharomyces cerevisiae isolated from contaminated wine in Yamanashi prefecture, Japan. We also found that the wild pellicle-forming strain had the deletion of the 111-bp repression sequence in the FLO11 promoter. Furthermore, we found that FLO8 gene encoding a transcriptional activator in the glucose signal transduction pathway is required for pellicle formation by the wild pellicle-forming strain. Based on the results obtained in this study, yeast or lactic acid bacteria strains which secrete inhibitors of Flo8p would be useful to develop a novel method for preventing deleterious pellicle formation in wineries.
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| Free Research Field |
応用微生物学
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