2013 Fiscal Year Final Research Report
Anaerobic degradation pathway of hetero-aromatic compounds
| Project/Area Number |
24780076
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Applied microbiology
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| Research Institution | University of Miyazaki |
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Principal Investigator |
INOUE Kengo 宮崎大学, IR推進機構, 助教 (70581304)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 鉄還元細菌 / 芳香族化合物 / ダイオキシン / 嫌気性細菌 / 環境汚染物質 / 分解微生物 / 環境浄化 / 分解系遺伝子 |
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| Research Abstract |
Anaerobic biodegradation of heterocyclic compounds, carbazole, dibenzofuran, and dibenzothiophene were investigated. Bacterial strains capable of degrading the heterocyclic compounds were isolated from enriched culture in which the heterocyclic compounds and insoluble Fe(III) oxide were added as the sole electron donor and accepter, respectively. Inocula were collected from river and dam sediments and soils. Among the enriched cultures, 10, 1, and 1 strains were isolated from the medium in which carbazole, dibenzofuran, and dibenzothiophene were used as the sole electron donor, respectively. 16S rDNA sequencing analysis revealed one of the carbazole-degrading isolates belongs to Desulfovibrionaceae. Its type strain could not degrade carbazole and, thus, their comparative genome analysis is now underway in order to identify the gene involved in carbazole degradation.
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[Journal Article] Structural basis of the divergent oxygenation reactions catalyzed by the Rieske non-heme oxygenase, carbazole 1,9a-dioxygenase2014
Author(s)
Inoue, K., Usami, Y., Ashikawa, Y., Noguchi, H., Umeda, T., Yamagami-Ashikawa, A., Horisaki, T., Uchimura, H., Terada, T., Nakamura, S., Shimizu, K., Yamane, H., Fujimoto, Z., Nojiri, H.
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Journal Title
Applied and Environmental Microbiology
Volume: 80巻
Pages: 2821-2832
DOI
Peer Reviewed
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[Journal Article] Uptake of self-secreted flavins as bound cofactors for extracellular electron transfer in Geobacter species2014
Author(s)
Okamoto, A., Saito, K., Inoue, K., Nealson, K., Hashimoto, K., Nakamura, R.
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Journal Title
Energy and Environmental Science
Volume: 7巻
Pages: 1357-1361
DOI
Peer Reviewed
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[Journal Article] Electricity generation from cattle manure slurry by cassette-electrode microbial fuel cells2014
Author(s)
Inoue, K., Ito, T., Kawano, Y., Iguchi, A., Miyahara, M., Suzuki, Y., Watanabe, K.
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Journal Title
Journal of Bioscience and Bioengineering
Volume: 12巻
Pages: 610-615
DOI
Peer Reviewed
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