2015 Fiscal Year Final Research Report
Novel extracellular function of molecular chaperones and mechanisms of biofilm formation in pathogenic bacteria
| Project/Area Number |
24780079
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | Jikei University School of Medicine |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2016-03-31
|
| Keywords | バイオフィルム / 分子シャペロン / ClpB / DnaK / マトリクス / 黄色ブドウ球菌 / 抗体 / 阻害剤 |
|---|
| Outline of Final Research Achievements |
Molecular chaperones play key roles in protein quality control in the cell. We found that cytoplasmic molecular chaperones ClpB and DnaK are incorporated into extracellular matrixes of Staphylococcus aureus and are involved in biofilm development. We here report that extracellularly excreted ClpB promotes biofilm formation in an oligomerization- and disaggregation activity-independent manner. Inhibition of these molecular chaperones using antibodies or small compounds leads to prevention of biofilms and persisters. These findings provide a novel mechanistic insight into molecular chaperones as well as a therapeutic strategy for chronic infectious diseases.
|
| Free Research Field |
微生物学
|
