Resequencing analyses to identify the genes for high temperature tolerance.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24780082
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied microbiology
• Research Institution: Tokyo University of Agriculture
• Principal Investigator: KANESAKI YU 東京農業大学, その他部局等, 博士研究員 (70380293)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: シアノバクテリア / 次世代シーケンサー / リシーケンス解析 / 突然変異 / 高温耐性

Outline of Final Research Achievements

Cyanobacteria are the photosynthetic microorganisms used as models for basic research on photosynthesis, evolution, and molecular genetics. Recent progress of sequencing technologies enables us to identify mutated loci in bacterial genomes. Here, we performed resequencing analysis of the genomes of five laboratory strains with different phenotypes on thermo-sensitivity. By verification of the identified mutations, we found a mutated locus which determined the thermo-sensitivity of the strains. Furthermore, we isolated the spontaneous thermo-tolerant mutants and identified a number of candidate loci for thermo-tolerance by resequencing analysis. Our strategy of genetics could efficiently identify the associated mutations in the spontaneous mutants which has altered phenotype.

Free Research Field

微生物学