2013 Fiscal Year Final Research Report
Characterization of mycobacterial novel monooxygenases and their application to biocatalysis
| Project/Area Number |
24780083
|
|---|
| Research Category |
Grant-in-Aid for Young Scientists (B)
|
| Allocation Type | Multi-year Fund |
|---|
| Research Field |
Applied microbiology
|
|---|
| Research Institution | Waseda University |
|---|
Principal Investigator |
|
|---|
| Project Period (FY) |
2012-04-01 – 2014-03-31
|
| Keywords | 酸化酵素 / 放線菌 / 異種発現 / シャペロニン / 生体触媒 |
|---|
| Research Abstract |
Bacterial binuclear iron monooxygenases play numerous physiological roles in oxidative metabolism. Monooxygenases of this type found in actinomycetes also catalyze various useful reactions and have attracted much attention as oxidation biocatalysts. However, difficulties in expressing these multicomponent monooxygenases in heterologous hosts have hampered the development of engineered oxidation biocatalysts. Here, we succeeded in functionally expressing the mycobacterial binuclear iron monooxygenase in heterologous hosts. We found that a specific chaperonin played essential roles in the active expression of the monooxygenase. The strategy developed here should be generally applicable to the heterologous expression of other actinomycetous binuclear iron monooxygenases, and will accelerate the development of engineered oxidation biocatalysts for industrial processes.
|
