Visualization of structure and function change of phosphorylatad protein.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24780092
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Tohoku University
• Principal Investigator: HIDAKA Masafumi 東北大学, (連合)農学研究科(研究院), 助教 (00584848)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: タンパク質工学

Outline of Final Research Achievements

Proteins are altered their structure by phosphorylation, and their functions are changed. Thus, the phosphorylation is key mechanism that involved in regulation of physiological functions. However, relationships between structural-switch and functional-switch by phosphorylation are still unclear. Elucidations of these relationships are expected to facilitate to elucidate mechanisms of phosphrylation-related diseases including cancer. We employed the structural switch of Pin1, that binds to phosphorylated proteins, to develop a novel detection system of phosphorylation-induced structural switch. Characterizations of the Pin1-dirived sensor protein indicated that this sensor protein can detect structural switch of Pin1 induced by phosphorylated sequence.

Free Research Field

タンパク質工学