Characterization and application of beta-glucan related enzymes

2014 Fiscal Year Final Research Report

• Project/Area Number: 24780099
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Applied biochemistry
• Research Institution: Mie University
• Principal Investigator: ISONO Naoto 三重大学, 生物資源学研究科, 准教授 (70378321)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: β-1,3-グルカン / ラミナリオリゴ糖 / 糖転移酵素 / スエヒロタケ / ホスホリラーゼ

Outline of Final Research Achievements

Some enzymes related to synthesis of beta-1,3-glucan were investigated in this study. The catalytic domain protein of beta-1,3-glucan synthase from Schizophyllum commune showed significant enzyme activity with UDPglucose. Glucosyltransferases were found in a cell wall autolysate and a culture medium of the fungal mycelia. In addition, novel bacterial beta-1,3-glucan phosphorylase and laminaridextrin phosphorylase were discovered and used for synthesis of beta-1,3-glucan and laminarioligosaccharides. Finally, water-soluble branched polysaccharide was synthesized in vitro using beta-1,3-glucan phosphorylase and bacterial glucosyltransferase.

Free Research Field

糖質科学・酵素化学・食品化学