The study of inhibitory mechanism of bovine milk lactophorin, which is independent of the binding to virion

2013 Fiscal Year Final Research Report

• Project/Area Number: 24780124
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Food science
• Research Institution: Gifu University
• Principal Investigator: INAGAKI Mizuho 岐阜大学, (連合)農学研究科(研究院), 学術研究補佐員 (50626356)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: ラクトフォリン / ヒトロタウイルス / 感染症 / 糖鎖 / 牛乳

Research Abstract

Human rotavirus (HRV) is a major etiologic agent of severe infantile gastroenteritis. In our previous study, we have found that bovine milk lactophorin (LP) exhibited inhibitory activity against HRV infection. In this study, we demonstrated that LP inhibits the synthesis and assembly of viral protein without contact between LP and virion. It indicated that LP has a unique inhibition mechanism against HRV infection.

Research Products

• [Journal Article] Bovine k-casein inhibits human rotavirus (HRV) infection via direct binding of glycans to HRV (2014)
  • Author(s): Inagaki, M., Muranishi, H., Yamada, K., kakehi, K., Uchida, K., Suzuki, T., Yabe, T., Nakagomi, T., Nakagomi, O., and Kanamaru Y.
  • Journal Title: J. Dairy Sci. Volume: 97(5) Pages: 2653-2661
  • DOI: 10.3168/jds.2013-7792
  • Peer Reviewed
• [Presentation] 牛乳ラクトフォリンによるロタウイルス非構造タンパク質NSP4遺伝子の転写抑制 (2013)
  • Author(s): 稲垣瑞穂、大野翔平、小林純子、高橋毅、杉山誠、金丸義敬、中込とよ子、中込治、鈴木徹
  • Organizer: 第61回日本ウイルス学会学術集会
  • Place of Presentation: 神戸
  • Year and Date: 20130000
• [Presentation] The utilization of concentrated sweet whey against human intestinal gastroenteritis (2013)
  • Author(s): Mizuho Inagaki, Yoshitaka Nakamura, Takeshi Takahashi, Tomio Yabe, Tohru Suzuki, Toyoko Nakagomi, Osamu Nakagomi, and Yoshihiro Kanamaru
  • Organizer: IDF World Dairy Summit2013
  • Place of Presentation: Yokohama
  • Year and Date: 20130000
• [Presentation] The inhibitory mechanism of bovine lactophorin against human rotavirus gastroenteritis (2012)
  • Author(s): Mizuho Inagaki, Shohei Ohno, Keita Yamada, Takeshi Takahashi, Tsukasa Matsuda, Tomio Yabe, Tohru Suzuki, Makoto Sugiyama, Toyoko Nakagomi, Osamu Nakagomi, and Yoshihiro Kanamaru
  • Organizer: World Congress of Microbes 2012
  • Place of Presentation: Guangzhou, China
  • Year and Date: 20120000
• [Presentation] 牛乳ラクトフォリンの示すヒトロタウイルス感染阻害作用(続報)
  • Author(s): 大野翔平、稲垣瑞穂、山田佳太、矢部富雄、鈴木徹、高橋毅、杉山誠、中込とよ子、中込治、金丸義敬
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪