Food environment of cultured oyster in Matsushima Bay, Japan

2014 Fiscal Year Final Research Report

• Project/Area Number: 24780179
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: General fisheries
• Research Institution: Tohoku University
• Principal Investigator: NISHITANI Goh 東北大学, (連合)農学研究科(研究院), 助教 (70450781)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 宮城県松島湾 / 二枚貝幼生 / 種判別 / 餌料環境

Outline of Final Research Achievements

Totally 516 natural bivalve larvae collected from Matsushima Bay were isolated and identified by molecular methods. A multiplex PCR assay has been developed that enables simultaneous detection of seven bivalve larvae species on the basis of differences in the lengths of the PCR products. The gut contents were analyzed to clarify the potential food sources of the adult oyster. Using universal primer and gene cloning, sequences of suspended phytoplankton, such as diatom Thalassiosira spp. and dinoflagellate Scrippsiella trochoidea, were mainly detected with other sequences of Bolidophyceae, sea squirts, red algae, ciliates, and copepoda.

Free Research Field

海洋分子生態学