2013 Fiscal Year Final Research Report

Screening of candidate proteins related to susceptibility of fugu to its parasite

Research Project

Project/Area Number	24780182
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	General fisheries
Research Institution	The University of Tokyo
Principal Investigator	TASUMI Satoshi 東京大学, 農学生命科学研究科, 助教 (90359646)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	宿主特異性 / 2次元電気泳動 / MS/MS / トラフグ / 鰓
Research Abstract	A monogenean parasite Heterobothrium okamotoi parasitize gills of Takifugu rubripes, but not those of its relative species T. niphobles, suggesting that the parasite recognize molecules exclusively existing in gills of T. rubripes. To screen such molecules, 2D electrophoresis followed by MALDI-MS/MS analysis, and suppression subtractive hybridization (SSH) techniques were applied. By 2D electrophoresis, at least 34 species specific protein spots were found, among them 18 spots were identified. By SSH, so far 350 candidate sequences have been obtained. In this study, further screening by cell surface display has not yet been completed, however, multiple candidates have already been obtained and a various experimental problems to be solved became apparent. Knowledge obtained by this research mentioned above is helpful for further research on searching host-specificity determining molecules.