2013 Fiscal Year Final Research Report
Development of a rapid immunochromatographic assay for detection of Vibrio parahaemolyticus
| Project/Area Number |
24780206
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Fisheries chemistry
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| Research Institution | Osaka Prefectural Institute of Public Health |
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Principal Investigator |
SAKATA Junko 大阪府立公衆衛生研究所, その他部局等, 研究員 (30455547)
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 腸炎ビブリオ / イムノクロマト法 / 迅速同定法 / 品質管理 |
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| Research Abstract |
To rapidly and simply determine whether bacterial colonies growing on agar, represented Vibrio parahaemolyticus, an immunochromatographic assay (VP-ICA) using a generated couple of different monoclonal antibodies (designated MAb-VP34 and MAb-VP109) against V. parahaemolyticus F0F1-ATP synthase's delta subunit was developed. When cells of 124 V. parahaemolyticus strains were treated with a detergent and the resulting extracts were tested with the VP-ICA, they yielded positive results. In contrast, those of 62 non-V. parahaemolyticus species, including 93 different strains, did not. These results show that VP-ICA is highly specific for V. parahaemolyticus.
The VP-ICA procedure took 30 min, indicating that VP-ICA will greatly reduce labor and time required to identify V. parahaemolyticus colonies as compared with the conventional biochemical tests.
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