Evaluation of gene delivery mechanism based on quantitative visualization of cellular defense system against exogenous genes for high-efficiency gene therapy

2014 Fiscal Year Final Research Report

• Project/Area Number: 24790036
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Physical pharmacy
• Research Institution: National Institute of Advanced Industrial Science and Technology (2014); Institute of Physical and Chemical Research (2013); Hokkaido University (2012)
• Principal Investigator: SASAKI Akira 独立行政法人産業技術総合研究所, バイオメディカル研究部門, 研究員 (30580162)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: 遺伝子デリバリー / 蛍光イメージング / 1分子計測 / 画像相関 / 酵素活性

Outline of Final Research Achievements

The Purpose of this study is to visualize intracellular diffusion and degradation of exogenous genes quantitatively. We have newly developed raster image cross-correlation spectroscopy (ccRICS) setup. Degradation activity of DNA in living cells was successfully monitored as a movie through a direct measurement by the developed ccRICS system. Our result shows that the DNA degradation activity in living cells differs between cell lines. The achievement reveals a defense machinery against exogenous DNA invasion and determine its activity. The work will contribute a development of nucleic acid medicine and gene therapy.

Free Research Field

生物物理学