2013 Fiscal Year Final Research Report
Mechanisms underlying the vulnerability of striatal dopaminergic neurons caused by stress-related mental disorders.
| Project/Area Number |
24790096
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Biological pharmacy
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| Research Institution | Hokuriku University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | パーキンソン病 / ストレス性精神障害 / ドパミン神経 / MPTP / シナプトソーム |
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| Research Abstract |
In order to establish the functional assay system for dopaminergic terminals in striatal synaptosomes, we use the fluorescent compound 4-(4-diethylaminostyryl)-N-methylpyridinium iodide (ASP+). ASP+ has been used to measure the biophysical properties of norepinephrine transporter (NET), serotonin transporter (SERT) or dopamine transporter (DAT). In this study, we have investigated whether ASP+ can be used as a fluorescent substrate to monitor DAT function in mouse striatal synaptosomes. We confirmed the specificity of ASP+ uptake for the NET, SERT and DAT using several inhibitors of monoamine transporters in synaptosomes prepared from the striatum and frontal cortex. These results suggest that the ASP+ method might be used to evaluate for the function of dopaminergic terminals in striatal synaptosomes.
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