development of the universal inducing agent for induction of antivirus cytotoxic T lymphocytes

2013 Fiscal Year Final Research Report

• Project/Area Number: 24790169
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Medical pharmacy
• Research Institution: Saitama Medical University
• Principal Investigator: KAWANO Masaaki 埼玉医科大学, 医学部, 助教 (30447528)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 薬学 / 医療薬剤学 / 免疫学 / ワクチン / 細胞傷害性T細胞 / SV40 / ウイルス様粒子 / VP1

Research Abstract

Cytotoxic T lymphocyte (CTL) has a role to eliminate virus-infected cells or cancer cells from the body. We developed novel universal inducing agent for antiviral CTL production using VP1 capsid protein of simian virus 40 (SV40). CTL peptide of HLA-A*02:01 against influenza A virus matrix protein 1 (M1), GILGFVFTL (FMP:58-66), was inserted into DE or HI loop region of VP1 which is exposed on the surface of the SV40 VP1 capsid. Purified FMP:58-66 inserted recombinant VP1 was immunized to HLA-A2 transgenic mice. As expected, lymphocyte of immunized mice contained CTL that responded against FMP:58-66 specifically. It indicated that VP1-based universal inducing agent could induce aimed CTL without need of adjuvant. By validating the molecular mechanism how VP1-based agent efficiently induce aimed CTL and the efficacy for cancer in vivo in experimental level more, VP1-based agent will be developed to eliminate virus infected cells or cancer cells from the human body for practical use.

Research Products

• [Journal Article] Chimeric SV40 virus-like particles induce specific cytotoxicity and protective immunity against influenza A virus without the need of adjuvants (2014)
  • Author(s): Kawano M, Morikawa K, Suda T, Ohno N, Matsushita S, Akatsuka T, Handa H, Matsui M
  • Journal Title: Virology Volume: 448 Pages: 159-167
  • DOI: 10.1016/j.virol.2013.10.010
  • Peer Reviewed
• [Journal Article] Viral protein-coating of magnetic nanoparticles using simian virus 40 VP1 (2013)
  • Author(s): Enomoto T , Kawano M , Fukuda H, Sawada W, Inoue T, Haw KC, Kita Y, Sakamoto S, Yamaguchi Y, Imai T, Hatakeyama M, Saito S, Sandhu A, Matsui M, Aoki I, Handa H
  • Journal Title: J Biotechnol. Volume: 167 Pages: 8-15
  • DOI: 10.1016/j.jbiotec.2013.06.005
  • Peer Reviewed
• [Journal Article] SV40 virus-like particles as an effective delivery system and its application to a vaccine carrier (2013)
  • Author(s): Kawano M, Matsui M, Handa H
  • Journal Title: Expert Rev Vaccines Volume: 12 Pages: 199-210
  • DOI: 10.1586/erv.12.149
  • Peer Reviewed
• [Journal Article] インフルエンザウイルス特異的CTLの誘導を増強するプラットフォームの開発 (2013)
  • Author(s): 川野雅章、松井政則、禾泰壽、半田宏
  • Journal Title: 埼玉医科大学雑誌 Volume: 40巻 Pages: 68
• [Journal Article] ウイルスコンポーネントを用いた新規DDS担体 (2012)
  • Author(s): 川野雅章、半田宏
  • Journal Title: Medical Science Digest Volume: 38巻 Pages: 2-3
• [Journal Article] インフルエンザウイルス特異的CTLの誘導を増強するプラットフォームの開発 (2012)
  • Author(s): 川野雅章、松井政則、禾泰壽、半田宏
  • Journal Title: 埼玉医科大学雑誌 Volume: 39巻 Pages: 9-13
• [Presentation] Development of a CTL-based vaccine carrier with self-adjuvant properties using simian virus 40 virus-like particles (2013)
  • Author(s): KAWANO Masaaki, MORIKAWA Katsuma, SUDA Tatsuya, OHNO Naohito, MATSUSHITA Sho, AKATSUKA Toshitaka, HANDA Hiroshi, MATSUI Masanori
  • Organizer: Annual meeting of the Japanese society for immunology 2013
  • Place of Presentation: Makuhari Messe, Chiba, Japan
  • Year and Date: 20131211-13
• [Presentation] ウイルス特異的CTLの誘導を増強するプラットフォームの開発 (2013)
  • Author(s): 川野雅章、松下祥、赤塚俊隆、半田宏、松井政則
  • Organizer: 第11回RCGMフロンティアシンポジウム
  • Place of Presentation: 埼玉医科大学日高キャンパス、創立30周年記念講堂
  • Year and Date: 20131122-23
• [Presentation] Chimeric VP1 of simian virus 40 induces IL-12 production from DC, facilitating CTL induction against an inserted CTL epitope within the VP1 (2012)
  • Author(s): KAWANO Masaaki, SUDA Tatsuya, MATSUSHITA Sho, AKATSUKA Toshitaka, HANDA Hiroshi, MATSUI Masanori
  • Organizer: Annual meeting of the Japanese society for immunology 2012
  • Place of Presentation: Kobe International Conference Center, Kobe International Exhibition Hall, Kobe Portopia Hotel, Hyogo, Japan
  • Year and Date: 20121205-07
• [Book] Virus-like Particles in Vaccine Development (2014)
  • Author(s): Masaaki Kawano, Masanori Matsui, Hiroshi Handa
  • Total Pages: 137
  • Publisher: Future Medicine
• [Patent(Industrial Property Rights)] 細胞傷害性T細胞誘導剤 (2012)
  • Inventor(s): 半田 宏、川野 雅章、松井 政則
  • Industrial Property Rights Holder: LSIP
  • Industrial Property Rights Type: 特許
  • Industrial Property Number: PCT/JP2012/60910
  • Filing Date: 2012-04-24
  • Overseas