2013 Fiscal Year Final Research Report

A mechanisum for Regulatory T cell induction by DST, Invivo immunohistological analysis.

Research Project

Project/Area Number	24790200
Research Category	Grant-in-Aid for Young Scientists (B)
Allocation Type	Multi-year Fund
Research Field	General anatomy (including Histology/Embryology)
Research Institution	Dokkyo Medical University
Principal Investigator	KITAZAWA Yusuke 獨協医科大学, 医学部, 助教 (00467581)
Project Period (FY)	2012-04-01 – 2014-03-31
Keywords	制御性T細胞 / ethynyl deoxyuridine / 細胞増殖 / 移植免疫応答 / ドナー特異的輸血 / フェノタイプ解析 / 多重免疫染色 / 細胞相互作用
Research Abstract	It is known that Donor-specific blood transfusion(DST) and additional organ transplantation induces antigen-specific regulatory T cells (iTreg). However, its mechanism is still unknown. We examined which blood components could induce this effect and its mechanism. We found that, T-cells in the blood could induce alloresponse most efficiently in the PALS, because the trafficking pattern of recirculating T-cells in the spleen were designed to freely enter the PALS and cluster with the resident DCs, thus having a chance to provide alloantigen to DCs. This study has revealed the precise mechanism for first time. T-cells pulsed with antigens might be applicable as a vaccine vector, being efficiently targeted on the resident DCs for the prophylactic antibody production.