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2013 Fiscal Year Final Research Report

Investigation of Baf53a and Oct3/4 in the self-renewal of ES cells

Research Project

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Project/Area Number 24790274
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field General medical chemistry
Research InstitutionKanazawa University

Principal Investigator

AKAGI TADAYUKI  金沢大学, 医学系, 助教 (70532183)

Project Period (FY) 2012-04-01 – 2014-03-31
KeywordsBaf53a / Oct3/4 / クロマチン・リモデリング
Research Abstract

Several transcription factors are involved in the self-renewal of ES cells. In the present study, I examined relationships between Oct3/4 (transcription factor) and Baf53a (chromatin remodeling factor). Baf53a mRNA expressed in the undifferentiated ES cells, and decreased after Oct3/4 repression. Also, Baf53a associated with the POU domain of Oct3/4. Loss of function experiment of Baf53a by RNAi revealed that Baf53a was involved in proliferation and acetylation of histon H4. Taken together, these results suggest that Baf53a, together with Oct3/4, is involved in proliferation and epigenomic maintenance of ES cells.

  • Research Products

    (13 results)

All 2014 2013 2012 Other

All Journal Article (4 results) (of which Peer Reviewed: 4 results) Presentation (8 results) Remarks (1 results)

  • [Journal Article] High Oxygen Condition Facilitates the Differentiation of Mouse and Human Pluripotent Stem Cells into Pancreatic Progenitors and Insulin-Producing Cells2014

    • Author(s)
      Hakim F, Kaitsuka T, Raeed JM, Wei FY, Shiraki N, Akagi T, Yokota T, Kume S, and Tomizawa K
    • Journal Title

      J Biol. Chem

      Volume: 289 Pages: 9623-38

    • Peer Reviewed
  • [Journal Article] The stem cell transcription factor ZFP57 induces IGF2 expression to promote anchorage-independent growth in cancer cells2014

    • Author(s)
      Tada Y, Yamaguchi Y, Kinjo T, Song X, Akagi T, Takamura H, Ohta T, Yokota T, and Koide H
    • Journal Title

      Oncogene

      Volume: (in press)

    • Peer Reviewed
  • [Journal Article] Zfp296 is a novel Klf4-interacting protein and functions as a negative regulator2013

    • Author(s)
      Fujii Y, Kakegawa M, Koide H, Akagi T, and Yokota T
    • Journal Title

      Biochem Biophys Res Commun

      Volume: 441 Pages: 411-417

    • Peer Reviewed
  • [Journal Article] Dax1 Associates with Esrrb and Regulates Its Function in Embryonic Stem Cells2013

    • Author(s)
      Uranishi K, Akagi T, Sun C, Koide H, and Yokota T
    • Journal Title

      Mol Cell Biol

      Volume: 33 Pages: 2056-66

    • Peer Reviewed
  • [Presentation] ES細胞における多分化能と増殖能における転写因子ETV4/5の関与2013

    • Author(s)
      赤木紀之, Kuure Satu, 小出寛, Costantini Frank, 横田崇
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸・神戸ポートアイランド
    • Year and Date
      20131203-06
  • [Presentation] Dax1はEsrrbを抑制することによってES細胞の内胚葉系への分化を抑制する2013

    • Author(s)
      浦西洸介, 赤木紀之, 小出寛, 横田崇
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸・神戸ポートアイランド
    • Year and Date
      20131203-06
  • [Presentation] Zfp296を介したKlf4の機能制御機構の解析2013

    • Author(s)
      藤井優佳, 懸川まどか, 小出寛, 赤木紀之, 横田崇
    • Organizer
      第36回日本分子生物学会
    • Place of Presentation
      神戸・神戸ポートアイランド
    • Year and Date
      20131203-06
  • [Presentation] マウスES細胞におけるLRH-1の増殖制御機構の解析2012

    • Author(s)
      金井大, 武石健, 上田篤, 赤木紀之, 小出寛, 横田崇
    • Organizer
      第85回日本生化学会大会
    • Place of Presentation
      福岡県・福岡国際会議場
    • Year and Date
      20121214-16
  • [Presentation] Zfp296のマウスES細胞における役割2012

    • Author(s)
      藤井優佳, 懸川まどか, 赤木紀之, 小出寛, 横田崇
    • Organizer
      第35回日本分子生物学会
    • Place of Presentation
      福岡・福岡国際会議場
    • Year and Date
      20121211-14
  • [Presentation] INVOLVEMENT OF ETS-RELATED TRANSCRIPTION FACTORS ETV4 AND ETV5 IN PLURIPOTENCY AND PROLIFERATION OF MOUSE EMBRYONIC STEM CELLS2012

    • Author(s)
      Tadayuki Akagi, Satu Kuure, Hiroshi Koide, Frank Costantini, and Takashi Yokota
    • Organizer
      ISSCR 10th Annual Meeting
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      20120613-16
  • [Presentation] DAX1 ASSOCIATES WITH ESRRB AND FUNCTIONS AS A REPRESSOR IN EMBRYONIC STEM CELLS2012

    • Author(s)
      Kousuke Uranishi, Tadayuki Akagi, Chuanhai Sun, Hiroshi Koide, and Takashi Yokota
    • Organizer
      ISSCR 10th Annual Meeting
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      20120613-16
  • [Presentation] NANOG/ZFP57 PATHWAY PROMOTES ANCHORAGE INDEPENDENT GROWTH OF HT1080 CELLS BY INDUCING IMPRINTED GENES EXPRESSION2012

    • Author(s)
      Tada Yuhki, Tadayuki Akagi, Takashi Yokota, and Hiroshi Koide
    • Organizer
      ISSCR 10th Annual Meeting
    • Place of Presentation
      Yokohama, Japan
    • Year and Date
      20120613-16
  • [Remarks]

    • URL

      http://medstem.w3.kanazawa-u.ac.jp/

URL: 

Published: 2015-06-25  

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