2014 Fiscal Year Final Research Report
The function of glycine N-methyltransferase in the proliferation of renal cell carcinoma
| Project/Area Number |
24790351
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Human pathology
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| Research Institution | Kyushu University |
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Principal Investigator |
SONG YooHyun 九州大学, 医学(系)研究科(研究院), 研究員 (10586746)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 腎癌 |
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| Outline of Final Research Achievements |
We have reported that the GNMT gene acts as an oncogene. However, little is known about the specific function of GNMT in carcinogenesis and progression in renal cell carcinoma (RCC).
We performed Western blotting analysis using normal kidney cells and RCC cells. GNMT proteins were expressed in normal kidney cell and most RCC cells.In immunohistochemistry, GNMT protein was expressed in the cytoplasm of RCC cells. We found that the knockdown of GNMT expression induced apparently effect on RCC cell proliferation. Furthermore, GNMT knockdown in RCC cell induced prominent increases in the sub-G1 fraction, indicating cellular death. To investigate the mechanism of cellular death by GNMT knockdown, Western blotting using an antibody against cleaved PARP, which indicates the presence of degradation products from the caspase cascade, was employed. GNMT knockdown induced cleavage of PARP in RCC cells.
GNMT may play an important role in promoting cell growth via the regulation of apoptosis of RCC.
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| Free Research Field |
医学
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