Acquisition of novel bio-markers for cancers using cancer-specific exosomal proteins.

2014 Fiscal Year Final Research Report

• Project/Area Number: 24790360
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Human pathology
• Research Institution: Kitasato University
• Principal Investigator: NAGASHIO Ryo 北里大学, 医療衛生学部, 講師 (40618568)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: エクソゾーム / ランダム免疫法 / 単クローン性抗体 / プロテオミクス

Outline of Final Research Achievements

There are few useful biomarkers for early diagnosis of lung cancer. Therefore most patients with lung cancer are at an advanced stage at the time of diagnosis. To obtain useful diagnostic markers for lung adenocarcinoma, we generated monoclonal antibodies using exosomal proteins from lung adenocarcinoma-derived A549 cells by employing the random immunization method. To evaluate the utility of these antibodies as differential diagnostic marker and/or sero-diagnostic marker for lung adenocarcinoma, we performed immunohistochemical study with tissue microarray and dotblot analysis with sera from lung cancer patients and normal controls. As the results, we successfully obtained the candidate biomarkers for diagnosis of lung adenocarcinomas.

Free Research Field

疾患プロテオミクス