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2013 Fiscal Year Final Research Report

Analysis of the autoantibodies produced in the diseased synovial tissue in rheumatoid arthritis: The application of the enzyme-labeled antigen method

Research Project

  • PDF
Project/Area Number 24790368
Research Category

Grant-in-Aid for Young Scientists (B)

Allocation TypeMulti-year Fund
Research Field Human pathology
Research InstitutionFujita Health University

Principal Investigator

MIZUTANI Yasuyoshi  藤田保健衛生大学, 医学部, 助教 (10546229)

Project Period (FY) 2012-04-01 – 2014-03-31
Keywords酵素抗原法 / 抗原特異的抗体産生細胞 / 関節リウマチ / コムギ胚芽無細胞蛋白合成系 / AlphaScreen
Research Abstract

The enzyme-labeled antigen method is a histochemical technique that visualizes antigen-specific antibody producing cells in tissue sections with labeled antigens as probes. In the present study, we attempted to visualize the site of autoantibody production in the rheumatoid synovium with the enzyme-labeled antigen method. The plasma cells producing atuoantibodies to human IgG were visualized in the synovial tissue sections. On the other hand, in the tissue sections of the lymph nodes of rats immunized with human autoantigens (FKBP4 and MAP1LC3B), the plasma cells producing antibodies to the immunized-antigens were visualized. But, in the synovial tissue, no positive cells were detected. These results suggest there are no plasma cells producing antibodies to autoantigens focused in the present study in the synovial tissues. Further studies are needed to demonstrate the plasma cells producing antibodies to the other autoantigens in the diseased synovial tissues.

  • Research Products

    (5 results)

All 2013 2012 Other

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (3 results) Remarks (1 results)

  • [Journal Article] Novel approach to identifying autoantibodies in rheumatoid synovitis with a biotinylated human autoantigen library and the enzyme-labeled antigen method2013

    • Author(s)
      Mizutani Y, Matsuoka K, Takeda H, Shiogama K, Inada K, Hayakawa K, Yamada H, Miyazaki T, Sawasaki T, Endo Y, Yutaka Tsutsumi
    • Journal Title

      J Immunol Method

      Volume: 387 Pages: 57-70

    • Peer Reviewed
  • [Presentation] 「酵素抗原法」~ビオチン標識抗原で特異抗体産生細胞の局在をみる!2013

    • Author(s)
      堤寛、水谷泰嘉
    • Organizer
      第54回日本組織細胞化学会総会・学術集会
    • Place of Presentation
      航空会館(東京都)
    • Year and Date
      20130927-28
  • [Presentation] 感染病変局所に浸潤する特異抗体産生細胞の解析 : 酵素抗原法による抗病原菌抗体産生形質細胞の局在証明2013

    • Author(s)
      水谷泰嘉、塩竈和也、尾之内高慶、稲田健一、堤寛
    • Organizer
      第102回日本病理学会総会
    • Place of Presentation
      ロイトン札幌(札幌市)
    • Year and Date
      20130606-08
  • [Presentation] Visualization of Plasma Cells Producing Disease-specific Antibodies with the Enzyme-labeled Antigen Method2012

    • Author(s)
      Mizutani Y, Shiogama K, Onouchi T, Inada KI, Tsutsumi Y
    • Organizer
      14th International Congress of Histochemistry and Cytochemistry (ICHC 2012)
    • Place of Presentation
      Kyoto, Japan. Young Histochemist award
    • Year and Date
      20120826-29
  • [Remarks] 藤田保健衛生大学医学部第一病理学ホームページ

    • URL

      http://info.fujita-hu.ac.jp/pathology1/

URL: 

Published: 2015-06-25  

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