Molecular mechanism during development of bleomycin-induced pulmonary fibrosis model

2013 Fiscal Year Final Research Report

• Project/Area Number: 24790374
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Experimental pathology
• Research Institution: The University of Tokyo
• Principal Investigator: SHIMAOKA TAKESHI 東京大学, 医学(系)研究科(研究院), 助教 (90422279)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 線維症 / 炎症

Research Abstract

We isolated CD45(-)CD31(-)Ter-119(-)GFP(-)PDGFR(+)ScaI(+)Mesenchymal stem cells (MSC) from Col-GFP Tg mice bone marrow. We established GFP-positive fibroblast differentiation from MSC by CTGF, bFGF and ascorbic acid. We pared down the candidates by transcriptome analysis and confirmed the involvement in fibroblast differentiation. We observed that several genes play an important role in fibroblast differentiation from MSC.

Research Products

• [Journal Article] Qualitative Rather than Quantitative Changes Are Hallmarks of Fibroblasts in Bleomycin-Induced Pulmonary Fibrosis (2013)
  • Author(s): Tatsuya Tsukui, Satoshi Ueha, Jun Abe, Shin-ichi Hashimoto, Shigeyuki Shichino, Takeshi Shimaoka, Francis H.W. Shand, Yasuka Arakawa, Kenshiro Oshima, Masahira Hattori, Yutaka Inagaki, Michio Tomura, Kouji Matsushima
  • Journal Title: Am J Pathol Volume: 183(3) Pages: 758-73
  • DOI: 10.1016/j.ajpath.2013.06.005
• [Journal Article] Distinct Roles for CXCR6+ and CXCR6- CD4+ T Cells in the Pathogenesis of Chronic Colitis (2013)
  • Author(s): Yasushi Mandai, Daisuke Takahashi, Koji Hase, Yuuki Obata, Yukihiro Furusawa, Masashi Ebisawa, Tomoo Nakagawa, Toru Sato, Tatsuro Katsuno, Yasushi Saito, Takeshi Shimaoka, Osamu Yokosuka, Kotaro Yokote, Hiroshi Ohno
  • Journal Title: PLoS One Volume: 8(6) Pages: e65488
  • DOI: 10.1371/journal.pone.0065488)
• [Presentation] CXCL16 shows protective role in alpha-galactosylceramide suppression of pulmonary fibrosis development (2014)
  • Author(s): Shimaoka Takeshi, Arakawa Yasuka, Ueha Satoshi, Tsukui Tatsuya, Shichino Shigeyuki, Matsushima Kouji
  • Organizer: Keystone Symposia, Fibrosis : From bench to bedside
  • Place of Presentation: Colorado, USA
  • Year and Date: 20140323-27
• [Presentation] CXCL16 shows protective role in alpha-galactosylceramide suppression of pulmonary fibrosis development (2013)
  • Author(s): Shimaoka Takeshi, Arakawa Yasuka, Ueha Satoshi, Tsukui Tatsuya, Shichino Shigeyuki, Matsushima Kouji
  • Organizer: 第42回日本免疫学会学術集会
  • Place of Presentation: 千葉
  • Year and Date: 20131211-13