Genome-wide mutation analysis of malaria mutator: epidemiological and experimental study

2014 Fiscal Year Final Research Report

• Project/Area Number: 24790401
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Tokyo Women's Medical University
• Principal Investigator: HONMA Hajime 東京女子医科大学, 医学部, 助教 (10617468)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Keywords: マラリア / ミューテーター / Plasmodium / 分子疫学 / 薬剤耐性

Outline of Final Research Achievements

Detailed mutational trend of a mutator malaria parasite possessing proofreading deficient DNA polymerase δ was analyzed. The mutation spectrum was biased, and the base substitution of the parasite was influenced by sequence context. By using the mutator parasite, chloroquine resistant parasites were successfully generated. DNA sequencing analysis of genes involved in genome replication and/or stability with global Plasmodium falciparum DNA samples was also conducted, and fundamental information on the genetic polymorphism was obtained.

Free Research Field

寄生虫学