2013 Fiscal Year Final Research Report
Analysis of the T3SS1-dependent cytotoxicity of Vibrio parahaemolyticus
| Project/Area Number |
24790416
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 腸炎ビブリオ / ３型分泌装置 / エフェクター / 細胞死 |
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| Research Abstract |
Vibrio parahaemolyticus, a major food-borne pathogen, exhibits cytotoxicity that is dependent on its type III secretion system (T3SS1). Although a T3SS1 effector VepA plays a major role in the cytotoxicity, the mechanism was unknown.
In this study, a yeast genome-wide screen revealed that subunit c of V-ATPase is indispensable for the toxicity of VepA. Biochemical subcellular fractionation showed that VepA may be associated with lysosomes in infected cells. Although the toxicity of VepA was independent of the function of V-ATPases, VepA induces lysosomal membrane permeabilization and the leakage of contents from lysosomes. Therefore, these data suggest that the T3SS1 effector VepA targets subunit c of V-ATPase and induces the rupture of host cell lysosomes and subsequent cell death.
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[Journal Article] The Vibrio parahaemolyticus effector VopC mediates Cdc42-dependent invasion of cultured cells but is not required for pathogenicity in an animal model of infection2014
Author(s)
Okada R, Zhou X, Hiyoshi H, Matsuda S, Chen X, Akeda Y, Kashimoto T, Davis BM, Iida T, Waldor MK, Kodama T
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Journal Title
Cell Microbiol
Volume: 16
Pages: 938-947
DOI
Peer Reviewed
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