2014 Fiscal Year Final Research Report
Isolation and identification of TREM-1 binding proteins as candidate for TREM-1 ligands in neutrophil-like differentiated HL-60 cells
| Project/Area Number |
24790424
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Juntendo University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | TREM-1 / Ligand / 好中球 / マクロファージ / 好中球NETs |
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| Outline of Final Research Achievements |
TREM-1 (triggering receptor expressed on myeloid cells-1) plays a role in inflammation by augmenting inflammatory responses via the production of proinflammatory cytokines in macrophage. Although natural TREM-1 ligands have not been identified, flow cytometrical analyses of the binding of labeled-recombinant TREM-1 suggest that TREM-1 ligands are expressed on neutrophils. Recent studies have demonstrated that neutrophil extracellular traps (NETs) was released from neutrophil to activate macrophage. Here we attempted to identify a potential TREM-1 ligand in the components of NETs.
HEK293 cells lysate of over-expressing TREM-1-GST fusion protein and nuclear extract from the neutrophil-like differentiated HL-60 cells were subjected to GST-pull down assay. As a result, about 72 kDa molecules were specifically bind to TREM-1. At present, these molecules were identified by mass spectrometric analysis, and measured ligand potency.
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| Free Research Field |
生化学
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