The study on the pathogenicity of Saffold virus by means of the identification of the receptor for infection

2013 Fiscal Year Final Research Report

• Project/Area Number: 24790449
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Virology
• Research Institution: Kanazawa Medical University
• Principal Investigator: HIMEDA Toshiki 金沢医科大学, 医学部, 准教授 (80340008)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 感染受容体 / Saffold virus

Research Abstract

The aim of the study is to identify the receptor for Saffold virus (SAFV) which is a novel human cardiovirus with unknown pathogenicity. Although 94 genes were selected as a candidate of the receptor for SAFV infection, the expression of those genes in the non-susceptible cells was unable to induce the infection of SAFV, suggesting that those genes may not be the receptor for SAFV infection. However, through the selection process, the virus binding assay demonstrated that the expression of the receptor for SAFV infection was changeable depending on the cell culture conditions. This phenomenon will be helpful to identify the receptor for SAFV infection.

Research Products

• [Journal Article] Role(s) of Leader protein of Saffold virus (2014)
  • Author(s): Shimizu A, Himeda T, Okuwa T, Muraki Y, Ohara Y
  • Journal Title: J. Clinic. Exp. Neuroimmunol Volume: (Article first published online)
  • DOI: 10.1111/cen3.12109
  • Peer Reviewed
• [Journal Article] Possibility of neuropathogenesis of Saffold virus suggested from the in vitro virus persistence (2013)
  • Author(s): Himeda T, Okuwa T, Muraki Y, Ohara Y
  • Journal Title: J. Clinic. Exp. Neuroimmunol Volume: 4 Pages: 163–164
  • DOI: 10.1111/cen3.12033
  • Peer Reviewed
• [Journal Article] Saffold virus type 3 (SAFV-3) (2013)
  • Author(s): Himeda T, Hosomi T, Okuwa T, Muraki Y, Ohara Y
  • DOI: 10.1371/journal.pone.0053194
• [Journal Article] 新しいピコルナウイルス感染症-ヒトカルジオウイルスを中心に- (2013)
  • Author(s): 大原義朗、姫田敏樹
  • Journal Title: 小児感染免疫 Volume: 25 Pages: 446-451
  • URL: http://www.jspid.jp/journal/full/02504/025040446.pdf
  • Peer Reviewed
• [Journal Article] Saffold virus, a novel human cardiovirus with unknown pathogenicity (2012)
  • Author(s): Himeda T, Ohara Y
  • Journal Title: J. Virol Volume: 86 Pages: 1292-1296
  • DOI: 10.1128/JVI.06087-11
  • Peer Reviewed
• [Presentation] Saffoldウイルス(SAFV)Leader蛋白のIFN産生抑制能の解析 (2013)
  • Author(s): 姫田敏樹、大桑孝子、村木靖、大原義朗
  • Organizer: 第25回日本神経免疫学会
  • Place of Presentation: 海峡メッセ、下関市
  • Year and Date: 20131127-29
• [Presentation] カルジオウイルスの神経病原性～Theilerウイルスとの比較から考えるSaffoldウイルスの病原性～ (2013)
  • Author(s): 姫田敏樹
  • Organizer: 第61回日本ウイルス学会学術集会(シンポジウム)
  • Place of Presentation: 神戸国際会議場、神戸市
  • Year and Date: 20131110-12
• [Presentation] Saffoldウイルスのin vitroにおける持続感染機序の解明 (2013)
  • Author(s): 姫田敏樹、大桑孝子、村木靖、大原義朗
  • Organizer: 第50回細菌学会中部支部会
  • Place of Presentation: ホテル竹島、蒲郡市
  • Year and Date: 20131018-19
• [Presentation] Saffoldウイルスの持続感染と神経病原性の可能性 (2013)
  • Author(s): 大原義朗、姫田敏樹、大桑孝子、村木靖、西山修平、高橋利幸、藤盛寿一、三須建郎、中島一郎、藤原一男、糸山泰人、青木正志、石崎義人、原寿郎、中村龍文
  • Organizer: 第18回日本神経感染症学会
  • Place of Presentation: シーガイアコンベンションセンター、宮崎市
  • Year and Date: 20131011-12
• [Presentation] 小児下痢症から分離される"Saffold virus"の病原性解析～持続感染機序の解明とSaffold virus陽性となった剖検例の考察～ (2013)
  • Author(s): 姫田敏樹、大桑孝子、村木靖、大原義朗
  • Organizer: 平成25年度北陸腸内細菌研究会
  • Place of Presentation: 福井県国際交流会館、福井市
  • Year and Date: 2013-07-13
• [Presentation] In vitroにおけるSaffoldウイルスの持続感染 (2012)
  • Author(s): 姫田敏樹、大桑孝子、村木靖、大原義朗
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: 大阪国際会議場、大阪府
  • Year and Date: 20121113-15
• [Presentation] ヒトカルジオウイルスのin vitroにおける持続感染 (2012)
  • Author(s): 姫田敏樹、大桑孝子、村木靖、大原義朗
  • Organizer: 第49回細菌学会中部支部会
  • Place of Presentation: 金沢大学十全講堂、金沢市
  • Year and Date: 20121109-10
• [Presentation] Saffold virus感染と神経疾患の関係 (2012)
  • Author(s): 大原義朗、姫田敏樹、大桑孝子、村木靖、西山修平、高橋利幸、藤盛寿一、三須建郎、中島一郎、藤原一男、糸山泰人、青木正志、石崎義人、原寿郎、中村龍文
  • Organizer: 第17回日本神経感染症学会
  • Place of Presentation: ホテルルビノ京都堀川、京都府
  • Year and Date: 20121019-20
• [Presentation] カルジオウイルスの持続感染とIFN応答 (2012)
  • Author(s): 姫田敏樹、大桑孝子、村木靖、大原義朗
  • Organizer: 第24回日本神経免疫学会
  • Place of Presentation: 軽井沢プリンスホテルウエスト、軽井沢
  • Year and Date: 20120920-21
• [Presentation] Persistent infection of SAFV-3 in vitro (2012)
  • Author(s): Himeda T, Okuwa T, Muraki Y, Ohara Y
  • Organizer: 第16回日本神経ウイルス研究会
  • Place of Presentation: 国立感染症研究所、東京
  • Year and Date: 20120830-31
• [Presentation] Reverse genetics of Saffold virus (2012)
  • Author(s): Himeda T, Okuwa T, Muraki Y, Ohara Y
  • Organizer: The 17th in a series of biennial meetings of the European Study Group on Molecular Biology of Picornaviruses (Europic2012)
  • Place of Presentation: Saint-Raphaël, France
  • Year and Date: 20120603-07
• [Remarks]
  • URL: http://www.kanazawa-med.ac.jp/~microbio/