2014 Fiscal Year Final Research Report
Clinical marker hunting with informatics and mass spectrometry
| Project/Area Number |
24790548
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Laboratory medicine
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| Research Institution | Chiba University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | 臨床検査 / Immuno-MS / 免疫沈降 / 抗体 / セロタイピング |
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| Outline of Final Research Achievements |
Motoi Nishimura and colleagues combined mass spectrometry and immunoprecipitation (so-called Immuno-MS) using widely-used clinical test immunoassay reagents. As an example, we have shown successful ApoE serotyping using Immuno-MS.
ApoE serotyping is based on the correlation between ApoE genotype and isoform; it is therefore possible to determine the genotype from the blood ApoE isoform combination. Serotyping ApoE using mass spectrometry promises highly accurate results while requiring minimal amounts of blood and reagents, resulting in lower costs, which suggest that proteomic-based ApoE serotyping may eventually become a routine clinical laboratory test.
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| Free Research Field |
検査病態学
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