Development of pancreatic beta cell differentiation induction system of the human iPS cell for the morbidity analysis of the genetic diabetes MODY

2013 Fiscal Year Final Research Report

• Project/Area Number: 24790730
• Research Category: Grant-in-Aid for Young Scientists (B)
• Allocation Type: Multi-year Fund
• Research Field: Gastroenterology
• Research Institution: Osaka University
• Principal Investigator: KONNO Masamitsu 大阪大学, 医学(系)研究科(研究院), 助教 (80618207)
• Project Period (FY): 2012-04-01 – 2014-03-31
• Keywords: 糖尿病

Research Abstract

MODY patient (MODY1, 3, 5) derived pluripotent stem cells were established from patients derived fibroblast cells using sendai virus. It was shown that this patient derived iPS cells had the same level of pluripotency as healthy person derived iPS cells. Differentiation induction was carried out to the pancreatic beta cells using the healthy person the MODY patient derived iPS cells. There were no difference into gene expression and the level of insulin secretion because of the efficiency of differentiation induction to pancreatic beta cells was very low in vitro.
The healthy person and patient derived pancreatic beta cells were transplanted under the kidney membrane using the diabetes model mice and the improvement effect of the diabetes was investigated by blood sugar level measurement. Since the lapsed days after a transplant were short, the significant difference between a healthy person and a MODY patient was not accepted.

Research Products

• [Journal Article] Surgically Resected Human Tumors Reveal the Biological Significance of the Gastric Cancer Stem Cell Markers CD44 and CD26 (2014)
  • Author(s): Nishikawa, S., Konno, M., Hamabe, A., Hasegawa, S., Kano, Y., Fukusumi, T., Satoh, S., Takiguchi, S., Mori, M., Doki, Y., Ishii, H
  • Journal Title: Int. J. Oncol Volume: (in press)
• [Journal Article] CD10 as a novel marker of therapeutic resistance and cancer stem cells in head and neck squamous cell carcinoma. (2014)
  • Author(s): Fukusumi, T., Ishii, H., Konno, M., Yasui, T., Nakahara, S., Takenaka, Y., Yamamoto, Y., Nishikawa, S., Kano, Y., Ogawa, H., Hasegawa, S., Hamabe, A., Haraguchi, N., Doki, Y., Mori, M., Inohara, H
  • Journal Title: British Journal of Cancer Volume: (in press)
• [Journal Article] Human colorectal CD24+ cancer stem cells are susceptible to epithelial-mesenchymal transition (2014)
  • Author(s): Okano, M., Konno, M., Kano, Y., Kim, H., Kawamoto, K., Ohkuma, H., Haraguchi, N., Yokobori, T., Mimori, K., Yamamoto, H., Sekimoto, M., Doki, Y., Mori, M., Ishii, H
  • Journal Title: Int. J. Oncol Volume: (in press)
• [Journal Article] Reprogramming Using microRNA-302 Improves Drug Sensitivity in Hepatocellular Carcinoma Cells (2014)
  • Author(s): Koga, C., Kobayashi, S., Nagano, H., Tomimaru, Y., Hama, N., Wada, H., Kawamoto, K., Eguchi, H., Konno, M., Ishii, M., Doki, Y., Mori, M
  • Journal Title: Ann. Surg. Oncol Volume: (in press)
• [Journal Article] SCGB2A1 is a novel prognostic marker for colorectal cancer associated with chemoresistance and radioresistance (2014)
  • Author(s): Munakata, K., Uemura, M., Takemasa, I., Ozaki, M., Konno, M., Nishimura, J., Hata, T., Mizushima, T., Haraguchi, N., Noura, S., Ikenaga, M., Okamura, S., Fukunaga, M., Murata, K., Yamamoto, H., Doki, T., Mori, M
  • Journal Title: Int. J. Oncol Volume: 44 Pages: 1521-1528
• [Journal Article] Novel drug discovery system for cancer stem cells in human squamous cell carcinoma of the esophagus (2014)
  • Author(s): Kano, Y., Konno, M., Kawamoto, K., Tamari, K., Hayashi, K., Fukusumi, T., Satoh, T., Tanaka, S., Ogawa, K., Mori, M., Doki, Y., Ishii, H
  • Journal Title: Oncol. Rep Volume: 31 Pages: 1133-1138
• [Journal Article] Cells of origin of squamous epithelium, dysplasia and cancer in the head and neck region after bone marrow transplantation (2014)
  • Author(s): Kano, Y., Ishii, H., Konno, M., Yamasaki, M., Miyata, H., Nishikawa, S., Hamabe, A., Ogawa, H., Takahashi, H., Ohta, K., Hasegawa, S., Tanaka, K., Fukusumi, T., Otsuka, M., Kawamoto, K., Haraguchi, N., Fujimoto, R., Isobe, M., Tomita, Y., Matsuura, N., Takiguchu, S., Mori, M., Doki, Y
  • Journal Title: Int. J. Oncol Volume: 44 Pages: 443-450
• [Journal Article] CD90-(Thy-1-) high selection enhances reprogramming capacity of murine adipose-derived mesenchymal stem cells (2013)
  • Author(s): Kawamoto, K., Konno, M., Nagano, H., Nishikawa, S., Tomomaru, Y., Hama, N., Wada, H., Kobayashi, S., Eguchi, H., Tanemura, M., Ito, T., Doki, Y., Mori, M., Ishii, H
  • Journal Title: Dis. Markers Volume: 35 Pages: 573-579
• [Journal Article] Significance of INHBA expression in human colorectal cancer (2013)
  • Author(s): Okano, M., Yamamoto, H., Ohkuma, H., Kano, Y., Kim, H., Nishikawa, S., Konno, M., Kawamoto, K., Haraguchi, N., Takemasa, I., Mizushima, T., Ikeda, M., Yokobori, T., Mimori, K., Sekimoto, M., Doki, Y., Mori, M., Ishii, H
  • Journal Title: Oncol. Rep Volume: 30 Pages: 2903-2908
• [Journal Article] Jumonji/Arid1b (Jarid1b) protein modulates human esophageal cancer cell growth (2013)
  • Author(s): Kano, Y., Konno, M., Ohta, K., Haraguchi, N., Nishikawa, S., Kagawa, Y., Hamabe, A., Hasegawa, S., Ogawa, H., Fukusumi, T., Noguchi, Y., Ozaki, M., Kudo, T., Sakai, D., Satoh, T., Ishii, M., Mizohata, E., Inoue, T., Mori, M., Doki, Y., Ishii, H
  • Journal Title: Molecular and Clinical Oncology Volume: 4 Pages: 753-757
• [Journal Article] Adipose-Derived Mesenchymal Stem Cells and Regenerative Medicine (2013)
  • Author(s): Konno, M., Hamabe, A., Hasegawa, S., Ogawa, H., Fukusumi, T., Nishikawa, S., Ohta, K., Kano, Y., Ozaki, M., Noguchi, Y., Sakai, D., Kudoh, T., Satoh, T., Doki, Y., Mori, M., Ishii, H
  • Journal Title: Develop. Growth Different Volume: 55 Pages: 309-318
• [Journal Article] Aldehyde dehydrogenasehigh gastric cancer stem cells are resistant to chemotherapy (2013)
  • Author(s): Nishikawa, S., Konno, M., Hamabe, A., Hasegawa, S., Kano, Y., Ohta, K., Fukusumi, T., Ozaki, M., Noguchi, Y., Sakai, D., Kudo, T., Haraguchi, N., Satoh, T., Doki, Y., Mori, M., Ishii, H
  • Journal Title: Int. J. Oncol Volume: 42 Pages: 1473-1442
• [Journal Article] Knockdown of JARID1B induces cellular senescence in human colorectal cancer (2013)
  • Author(s): Ohta, K., Haraguchi, H., Nishikawa, S., Kano, Y., Kagawa, Y., Konno, M., Hamabe, A., Hasegawa, S., Ogawa, H., Fukusumi, T., Noguchi, Y., Ozaki, M., Kudo, T., Sakai, D., Satoh, T., Ishii, M., Yamamoto, H., Doki, D., Mori, M., Ishii, H
  • Journal Title: Int. J. Oncol Volume: 42 Pages: 1212-1218
• [Journal Article] Transcriptome study of dormant gastrointestinal cancer stem cells (2012)
  • Author(s): Nishikawa, S., Dewi, D.L., Ishii, H., Konno, M., Haraguchi, N., Kano, Y., Fukusumi, T., Ohta, K., Noguchi, Y., Ozaki, M., Sakai, D., Satoh, T., Doki, Y., Mori, M
  • Journal Title: Int. J. Oncol Volume: 41 Pages: 979-984
• [Presentation] Innovative microRNA-Based Cellular Reprogramming Medicine to Exterminate of Cancer Stem Cells (2013)
  • Author(s): Masamitsu Konno, Hisataka Ogawa1, Yuichiro Doki, Masaki Mori, Hideshi Ishii
  • Organizer: 9th International Symposium on Minimal Residual Cancer
  • Place of Presentation: France
  • Year and Date: 20130925-27
• [Presentation] 食道扁平上皮癌においてヒストン H3K4脱メチル化酵素 JARID1Bが増殖能、浸潤能を促進している (2013)
  • Author(s): 加納義浩、石井秀始、原口直紹、福角隆仁、尾崎みゆ希、今野雅允、佐藤太郎、土岐祐一郎、森正樹
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 20130900
• [Presentation] 癌幹細胞の不活化を目指した革新的マイクロ RNA創薬と消化器癌治療への応用 (2013)
  • Author(s): 今野雅允、小川久貴、山本剛史、西川晋平、加納義浩、佐藤太郎、山本浩文、西山伸宏、片岡一則、小比賀聡、土岐祐一郎、森正樹、石井秀始
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 20130900
• [Presentation] 食道扁平上皮癌におけるヒストン H3K4脱メチル化酵素 JARID1の発現制御効果 (2013)
  • Author(s): 加納義浩、今野雅允、西川晋平、福角隆仁、尾崎みゆ希、坂井大介、工藤敏弘、佐藤太郎、山本浩文、土岐祐一郎、森正樹、石井秀始
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 20130900
• [Presentation] 革新的癌細胞修飾テクノロジーのためのiPS細胞リプログラミング (2013)
  • Author(s): 川本弘一、永野浩昭、今野雅允、小川久貴、古賀睦人、三吉範克、富丸慶人、小林省吾、江口英利、山本剛史、山本浩文、土岐祐一郎、森正樹、石井秀始
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 20130900
• [Presentation] microRNAを用いた癌リプログラミングによる新規癌治療 (2013)
  • Author(s): 小川久貴、今野雅允、長谷川慎一郎、浜部敦史、西川晋平、加納義浩、福角隆仁、呉しん、宮崎進、山本浩文、西山伸宏、片岡一則、小比賀聡、土岐祐一郎、森正樹、石井秀始
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 20130900
• [Presentation] CD10(+)の頭頸部扁平上皮癌細胞は治療抵抗性であり、癌幹細胞の特質を持つ (2013)
  • Author(s): 福角隆仁、石井秀始、今野雅允、西川晋平、加納義浩、小川久貴、長谷川慎一郎、工藤敏啓、坂井大介、佐藤太郎、土岐祐一郎、森正樹、猪原秀典
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 20130900
• [Presentation] Innovative microRNA-Based Cellular Reprogramming Medicine for Extermination of Cancer Stem Cells (2013)
  • Author(s): Masamitsu Konno, Hisataka Ogawa, Tsuyoshi Yamamoto, Yuichiro Doki, Nobuhiro Nishiyama, Kazunori Kataoka, Satoshi Obika, Masaki Mori, Hideshi Ishii
  • Organizer: The 11th Stem Cell Research Symposium
  • Place of Presentation: 東京
  • Year and Date: 20130517-18
• [Presentation] Induced Pluripotent Stem Cell Reprogramming for Innovative Cell-Modifying Technology in Cancer (2013)
  • Author(s): Koichi Kawamoto, Hideshi Ishii, Masamitsu Konno, Hisataka Ogawa, Hidetoshi Eguchi, Masahiro Tanemura, Hiroaki Nagano, Yuichiro Doki, Masaki Mori
  • Organizer: The 11th Stem Cell Research Symposium
  • Place of Presentation: 東京
  • Year and Date: 20130517-18
• [Presentation] Innovative Bridged Nucleic Acid (BNA)-Based Cellular Reprogramming Medicine Towards Extermination of Gastrointestinal Cancer (2013)
  • Author(s): Masamitsu Konno, Hideshi Ishii, Norikatsu Miyoshi, Tsuyoshi Yamamoto, Nobuhiro Nishiyama, Hisataka Ogawa, Shimpei Nishikawa, Shinichiro Hasegawa, Katsuya Ohta, Yoshihiro Kano, Takahito Fukusumi, Atsushi Hamabe, Taroh Satoh, Yuichiro Doki, Kazunori Kataoka, Satoshi Obika, Masaki Mori
  • Organizer: American Association for Cancer Research Annual meeting 2013
  • Place of Presentation: Washington DC USA
  • Year and Date: 20130406-10
• [Presentation] Identification of microRNA networks in pancreatic cancer stem cells. (2013)
  • Author(s): Shinichiro Hasegawa, Hideshi Ishii, Shimpei Nishikawa, Hidetoshi Eguchi, Shogo Kobayashi, Hiroshi Wada, Naoki Hama, Hirofumi Akita, Koichi Kawamoto, Masamitsu Konno, Hisataka Ogawa, Katsuya Ohta, Yoshihiro Kano, Takahito Fukusumi, Atsushi Hamabe, Takenori Nishimura, Kunihiko Hinohara, Taroh Satoh, Noriko Gotoh, Yuichiro Doki, Masaki Mori, Hiroaki Nagano
  • Organizer: American Association for Cancer Research Annual meeting 2013
  • Place of Presentation: Washington DC USA
  • Year and Date: 20130406-10
• [Presentation] Surface marker screening revealed the marker distinguishes gastric cancer stem cells in CD44 negative population. (2013)
  • Author(s): Shimpei Nishikawa, Hideshi Ishii, Masamitsu Konno, Naotsugu Haraguchi, Yoshihiro Kano, Atsushi Hamabe, Shinichiro Hasegawa, Hisataka Ogawa, Takahito Fukusumi, Katsuya Ohta, Toshihiro Kudo, Daisuke Sakai, Shuji Takiguchi, Taroh Satoh, Masaki Mori, Yuichiro Doki
  • Organizer: American Association for Cancer Research Annual meeting 2013
  • Place of Presentation: Washington DC USA
  • Year and Date: 20130406-10
• [Presentation] An Impact of Epigenetic Approaches Targeting JARID1B to Modulate Malignant Behaviors of Gastrointestinal Cancer (2013)
  • Author(s): Katsuya Ohta, Yoshihiro Kano, Masamitsu Konno, Yoshinori Kagawa, Shimpei Nishikawa, Shinichiro Hasegawa, Takahito Fukusumi, Hisataka Ogawa, Atsushi Hamabe, Taroh Satoh, Naotsugu Haraguchi, Masaru Ishii, Hirofumi Yamamoto, Yuichiro Doki, Masaki Mori, Hideshi Ishii
  • Organizer: American Association for Cancer Research Annual meeting 2013
  • Place of Presentation: Washington DC USA
  • Year and Date: 20130406-10
• [Presentation] An Innovative Cell-Modifying Technology for Cancer Reprogramming (2013)
  • Author(s): Hisataka Ogawa, Hirofumi Yamamoto, Masamitsu Konno, Shin Kure, Susumu Miyazaki, Shimpei Nishikawa, Shinichiro Hasegawa, Katsuya Ohta, Yoshihiro Kano, Takahito Fukusumi, Atsushi Hamabe, Takeshi Yamamoto, Satoshi Obika, Taroh Satoh, Hidetoshi Eguchi, Hiroaki Nagano, Hidenori Inohara, Yuichiro Doki, Masaki Mori, Hideshi Ishii
  • Organizer: American Association for Cancer Research Annual meeting 2013
  • Place of Presentation: Washington DC USA
  • Year and Date: 20130406-10
• [Presentation] マイクロRNAを用いた癌幹細胞のリプログラミングによる癌治療 (2013)
  • Author(s): 今野雅允、小川久貴、尾崎みゆ希、野口裕子、土岐祐一郎、森正樹、石井秀始
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20130321-23
• [Presentation] 単一遺伝子異常による糖尿病 (MODY)患者由来ヒトiPS細胞の樹立 (2013)
  • Author(s): 矢部茂治、岩崎直子、浜崎辰夫、今野雅允、大河内仁志
  • Organizer: 第12回日本再生医療学会総会
  • Place of Presentation: 横浜
  • Year and Date: 20130321-23
• [Presentation] 骨髄内遊離癌細胞の転移能を左右するマイクロRNAの研究 (2013)
  • Author(s): 今野雅允、三森功士、岩谷岳、西川晋平、深川剛生、笹子三津留、土岐祐一郎、森正樹、石井秀始
  • Organizer: がん支援公開シンポジウム
  • Place of Presentation: 東京
  • Year and Date: 20130129-30
• [Presentation] 胃癌幹細胞の同定とその生物学的解析 (2012)
  • Author(s): 西川晋平、原口直紹、今野雅允、加納義浩、福角隆仁、太田勝也、浜部敦史、長谷川慎一郎、工藤敏啓、坂井大介、瀧口修司、佐藤太郎、土岐祐一郎、森正樹、石井秀始
  • Organizer: 第23回日本消化器癌発生学会総会
  • Place of Presentation: 徳島
  • Year and Date: 20121115-16
• [Presentation] Induced Pluripotent Stem Cells Reprogramming For Innovative Cell-Modifying Technology in Cancer (2012)
  • Author(s): Masamitsu Konno, Yuichiro Doki, Masaki Mori, Hideshi Ishii
  • Organizer: The 8th International Symposium on Cancer Research and Therapy Tokyo
  • Place of Presentation: Japan
  • Year and Date: 20121109-10
• [Presentation] An Innovative Stem Cell-Modifying Technology in Gastrointestinal Cancer. (2012)
  • Author(s): Hideshi Ishii, Naotsugu Haraguchi, Masamitsu Konno, Yuichiro Doki, Masaki Mori
  • Organizer: The 8th International Symposium on Cancer Research and Therapy Tokyo
  • Place of Presentation: Japan
  • Year and Date: 20121109-10
• [Presentation] Novel Approaches To Treat Gastrointestinal Cancers Using Induced Pluripotent Stem Cell Reprogramming Technology Cell (2012)
  • Author(s): Masamitsu Konno, Shimpei Nishikawa, Yuichiro Doki, Masaki Mori, Hideshi Ishii
  • Organizer: Symposia Hallmarks of Cancer
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 20121029-31
• [Presentation] Biological characterization of gastric cancer stem cell. (2012)
  • Author(s): Shimpei Nishikawa, Masamitsu Konno, Naotsugu Haraguchi, Yuichiro Doki, Masaki Mori, Hideshi Ishii
  • Organizer: Cell Symposia Hallmarks of Cancer
  • Place of Presentation: San Francisco, CA, USA
  • Year and Date: 20121029-31
• [Presentation] 胃癌幹細胞の同定とその生物学的解析 (2012)
  • Author(s): 西川晋平、石井秀始、原口直紹、加納義浩、福角隆仁、尾崎みゆ希、今野雅允、瀧口修司、浜部敦史、佐藤太郎、土岐祐一郎、森正樹
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 20120900
• [Presentation] 分化多能性誘導性マイクロRNA群の同定と消化器癌治療への応用 (2012)
  • Author(s): 今野雅允、原口直紹、古賀睦人、西川晋平、加納義浩、佐藤太郎、小林省吾、江口英利、山本浩文、永野浩昭、土岐祐一郎、森正樹、石井秀始
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌
  • Year and Date: 20120900