2014 Fiscal Year Final Research Report
Novel insights of the mechanism in uptake of modified-LDL in macrophage through phospholipase A2
| Project/Area Number |
24790749
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | University of Yamanashi |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Keywords | ホスホリパーゼA2 / 酸化LDL / Src / マクロファージ / ノックダウン / 過剰発現 / endosome |
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| Outline of Final Research Achievements |
We established a stable cell line which expresses low levels of group V sPLA2 with the use of shRNA-knockdown method in Raw264.7 mouse macrophage cell line. This cell line showed delayed- and decreased-activities in uptake and degradation of oxidized-LDL, and these cells also showed decreases of the level of actin polymerization and the activity of Rho GTPase which regulate the uptake of oxidized-LDL into the cells, in comparison with wild type cell line. The expression level and the enzymatic activity of Src were reduced in group V sPLA2-knockdown cell line, and these phenomenons suggested that group V sPLA2 interacts with the promoter region in Src and regulates the expression of Src.
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| Free Research Field |
基礎循環器病学
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