2013 Fiscal Year Final Research Report
Detection of Notch1 mutation using PCR-Clamp method
| Project/Area Number |
24790797
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| Research Category |
Grant-in-Aid for Young Scientists (B)
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| Allocation Type | Multi-year Fund |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Hokkaido University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2014-03-31
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| Keywords | 肺癌 |
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| Research Abstract |
I tried to develop a highly specific detection system of Notch 1 mutation. To amplify the point mutation of Notch 1, I used the PCR clamp method. First, I tried to a clamp primer which covers the normal DNA of the mutation site. Second, I tried to design a mutant probe which covers the mutation sequence. But we cannot get the adequate primer.
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